{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T18:15:06Z","timestamp":1760984106744},"reference-count":36,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2011,7,20]],"date-time":"2011-07-20T00:00:00Z","timestamp":1311120000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Magnetic Resonance Imaging"],"published-print":{"date-parts":[[2011,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Purpose:<\/jats:title><jats:p>To investigate the targeting activity of the peptide (named P1c) derived from connective tissue growth factor (CTGF) to \u03b1v\u03b23\u2010rich tumor cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods:<\/jats:title><jats:p>P1c was synthesized and conjugated with ultrasuperparamagnetic iron oxide particles (USPIOs) coated with meso\u20102,3\u2010dimercaptosuccinic acid (DMSA). The specific binding activity of P1c\u2010USPIOs to \u03b1v\u03b23 was verified by solid phase binding assay. The combination of P1c\u2010USPIOs with a human primary liver cancer cell (Bel 7402) with \u03b1v\u03b23\u2010positive expression and uptake of P1c\u2010USPIOs by cells was investigated by Prussian blue staining, transmission electron microscopy (TEM), and magnetic resonance imaging (MRI). The targeting activity of the probe in vivo was also evaluated using a small\u2010animal tumor model by MRI.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results:<\/jats:title><jats:p>The cell uptake of P1c\u2010USPIOs was observed in a dose\u2010dependent manner, whereas no significant particle uptake was found in the plain USPIOs group. The differences on T2*\u2010weighted imaging were also found by MRI and the signal intensity (SI) was statistically reduced after coculture of Bel 7402 cells with P1c\u2010USPIOs at a concentration of 20\u201380 \u03bcg\/mL compared with plain USPIOs (<jats:italic>P<\/jats:italic> &lt; 0.05). The in vivo study showed that the signal reduction was distributed mainly in the periphery and some central areas of the tumor. The tumor\u2010to\u2010muscle CNR (contrast\u2010to\u2010noise ratio) at 12 hours after the administration of the P1c\u2010USPIOs was statistically significantly different compared to those at 0 hour, 1 hour, or the plain USPIO group (<jats:italic>P<\/jats:italic> &lt; 0.05).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion:<\/jats:title><jats:p>The peptide P1c might be a good candidate as a targeting carrier for drugs or tracers. J. Magn. Reson. Imaging 2011;. \u00a9 2011 Wiley\u2010Liss, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jmri.22620","type":"journal-article","created":{"date-parts":[[2011,7,20]],"date-time":"2011-07-20T12:06:35Z","timestamp":1311163595000},"page":"395-402","source":"Crossref","is-referenced-by-count":13,"title":["Novel peptide targeting integrin \u03b1v\u03b23\u2010rich tumor cells by magnetic resonance 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