{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,29]],"date-time":"2026-01-29T23:31:58Z","timestamp":1769729518861,"version":"3.49.0"},"reference-count":37,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2015,7,31]],"date-time":"2015-07-31T00:00:00Z","timestamp":1438300800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000024","name":"Canadian Institutes for Health Research","doi-asserted-by":"crossref","award":["CIHR-MOP-209113"],"award-info":[{"award-number":["CIHR-MOP-209113"]}],"id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001804","name":"Canadian Research Chairs","doi-asserted-by":"crossref","award":["950-228038"],"award-info":[{"award-number":["950-228038"]}],"id":[{"id":"10.13039\/501100001804","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"crossref","award":["RGPIN-356310-2013"],"award-info":[{"award-number":["RGPIN-356310-2013"]}],"id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Ontario Research Fund","award":["RE03-051"],"award-info":[{"award-number":["RE03-051"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Magnetic Resonance Imaging"],"published-print":{"date-parts":[[2016,3]]},"abstract":"<jats:sec><jats:title>Purpose<\/jats:title><jats:p>To test the feasibility of hyperpolarized [1\u2010<jats:sup>13<\/jats:sup>C]pyruvate magnetic resonance imaging (MRI) for noninvasive examination of guinea pig fetoplacental metabolism and nutrient transport.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>Seven pregnant guinea pigs with a total of 30 placentae and fetuses were anesthetized and scanned at 3T. <jats:italic>T<jats:sub>1<\/jats:sub><\/jats:italic>\u2010weighted <jats:sup>1<\/jats:sup>H images were obtained from the maternal abdomen. An 80 mM solution of hyperpolarized [1\u2010<jats:sup>13<\/jats:sup>C]pyruvate (hereafter referred to as pyruvate) was injected into a vein in the maternal foot. Time\u2010resolved 3D <jats:sup>13<\/jats:sup>C images were acquired starting 10 seconds after the beginning of bolus injection and every 10 seconds after to 50 seconds. The pregnant guinea pigs were recovered after imaging. Regions of interest (ROIs) were drawn around the maternal heart and each placenta and fetal liver in all slices in the <jats:sup>1<\/jats:sup>H images. These ROIs were copied to the <jats:sup>13<\/jats:sup>C images and were used to calculate the sum of the pyruvate and lactate signal intensities for each organ. The signal intensities were normalized by the volume of the organ and the maximum signal in the maternal heart.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>No adverse events were observed in the pregnant guinea pigs and natural pupping occurred at term (\u223c68 days). Pyruvate signal was observed in all 30 placentae, and lactate, a by\u2010product of pyruvate metabolism, was also observed in all placentae. The maximum pyruvate and lactate signals in placentae occurred at 20 seconds. In addition to the observation of pyruvate and lactate signals in the placentae, both pyruvate and lactate signals were observed in all fetal livers. The maximum pyruvate and lactate signals in the fetal livers occurred at 10 seconds and 20 seconds, respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>This work demonstrates the feasibility of using hyperpolarized [1\u2010<jats:sup>13<\/jats:sup>C]pyruvate MRI to noninvasively examine fetoplacental metabolism and transport of pyruvate in guinea pigs. Hyperpolarized <jats:sup>13<\/jats:sup>C MRI may provide a novel method for longitudinal studies of fetoplacental abnormalities. J. Magn. Reson. Imaging 2015. J. MAGN. RESON. 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