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Good test\u2013retest reproducibility is key when assessing the efficacy of treatments aimed at modulating GSH levels within the central nervous system or when noninvasively assessing changes in GSH content over time.<\/jats:p><\/jats:sec><jats:sec><jats:title>Purpose<\/jats:title><jats:p>To evaluate the intraday (in vitro and in vivo) and 1\u2010month apart (in vivo) test\u2013retest reproducibility of GSH measurements from GSH\u2010edited MEGA\u2010PRESS acquisitions at 3\u2009T in a phantom and in the brain of a cohort of middle\u2010aged and older healthy volunteers.<\/jats:p><\/jats:sec><jats:sec><jats:title>Study Type<\/jats:title><jats:p>Prospective.<\/jats:p><\/jats:sec><jats:sec><jats:title>Subjects\/Phantoms<\/jats:title><jats:p>A phantom containing physiological concentrations of GSH and metabolites with overlapping spectral signatures and 10 healthy volunteers (4 F, 6 M, 55\u2009\u00b1\u200914\u2009years old).<\/jats:p><\/jats:sec><jats:sec><jats:title>Field strength\/Sequence<\/jats:title><jats:p>GSH\u2010edited spectra were acquired at 3\u2009T using the MEGA\u2010PRESS sequence.<\/jats:p><\/jats:sec><jats:sec><jats:title>Assessment<\/jats:title><jats:p>The phantom was scanned twice and the healthy subjects were scanned three times (on two separate days, 1\u2009month apart). GSH was quantified from each acquisition, with the in vivo voxels placed at the primary motor cortex (PMC) and the occipital cortex (OCC).<\/jats:p><\/jats:sec><jats:sec><jats:title>Statistical Tests<\/jats:title><jats:p>Mean coefficients of variation (CV) were used to assess short\u2010term (in vitro and in vivo) and longer\u2010term (in vivo) test\u2013retest reproducibility.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In vitro, the CV was 2.3%. In vivo, the mean intraday CV was 3.3% in the PMC and 2.4% in the OCC, while the CVs at 1\u2009month apart were 4.6% in the PMC and 7.8% in the OCC.<\/jats:p><\/jats:sec><jats:sec><jats:title>Data Conclusion<\/jats:title><jats:p>GSH\u2010edited MEGA\u2010PRESS spectroscopy allows measurement of GSH with excellent precision.<\/jats:p><\/jats:sec><jats:sec><jats:title>Evidence Level<\/jats:title><jats:p>1<\/jats:p><\/jats:sec><jats:sec><jats:title>Technical Efficacy<\/jats:title><jats:p>Stage 2<\/jats:p><\/jats:sec>","DOI":"10.1002\/jmri.27842","type":"journal-article","created":{"date-parts":[[2021,7,29]],"date-time":"2021-07-29T06:55:35Z","timestamp":1627541735000},"page":"435-442","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Assessment of the Precision in Measuring Glutathione at <scp>3\u2009T<\/scp> With a <scp>MEGA\u2010PRESS<\/scp> Sequence in Primary Motor Cortex and Occipital Cortex"],"prefix":"10.1002","volume":"55","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1356-4513","authenticated-orcid":false,"given":"Adriana","family":"Anton","sequence":"first","affiliation":[{"name":"Department of Infection Immunity &amp; Cardiovascular Disease, Medical School, Faculty of Medicine Dentistry and Health, University of Sheffield  Sheffield UK"},{"name":"Sheffield NIHR Biomedical Research Centre: Translational Neuroscience for Chronic Neurological Disorders  Sheffield UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard J.","family":"Mead","sequence":"additional","affiliation":[{"name":"Department of Neuroscience, School of Medicine Sheffield Institute for Translational Neuroscience, University of Sheffield  Sheffield UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pamela J.","family":"Shaw","sequence":"additional","affiliation":[{"name":"Sheffield NIHR Biomedical Research Centre: Translational Neuroscience for Chronic Neurological Disorders  Sheffield UK"},{"name":"Department of Neuroscience, School of Medicine Sheffield Institute for Translational Neuroscience, University of Sheffield  Sheffield UK"},{"name":"Sheffield Teaching Hospitals NHS Foundation Trust  Sheffield UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard A. E.","family":"Edden","sequence":"additional","affiliation":[{"name":"Russell H. Morgan Department of Radiology and Radiological Science The Johns Hopkins University School of Medicine  Baltimore Maryland USA"},{"name":"F. M. Kirby Research Center for Functional Brain Imaging Kennedy Krieger Institute  Baltimore Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julia","family":"Bigley","sequence":"additional","affiliation":[{"name":"Department of Infection Immunity &amp; Cardiovascular Disease, Medical School, Faculty of Medicine Dentistry and Health, University of Sheffield  Sheffield UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas M.","family":"Jenkins","sequence":"additional","affiliation":[{"name":"Department of Neuroscience, School of Medicine Sheffield Institute for Translational Neuroscience, University of Sheffield  Sheffield UK"},{"name":"Sheffield Teaching Hospitals NHS Foundation Trust  Sheffield UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7246-8660","authenticated-orcid":false,"given":"Jim M.","family":"Wild","sequence":"additional","affiliation":[{"name":"Department of Infection 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