{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,11]],"date-time":"2025-12-11T21:01:50Z","timestamp":1765486910014,"version":"3.40.5"},"reference-count":36,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2023,5,30]],"date-time":"2023-05-30T00:00:00Z","timestamp":1685404800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100021171","name":"Basic and Applied Basic Research Foundation of Guangdong Province","doi-asserted-by":"publisher","award":["2019A1515011143","2020A1515011332","2022A1515012503"],"award-info":[{"award-number":["2019A1515011143","2020A1515011332","2022A1515012503"]}],"id":[{"id":"10.13039\/501100021171","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Magnetic Resonance Imaging"],"published-print":{"date-parts":[[2024,2]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>The promoter variant rs17111237 in the <jats:italic>CEP128<\/jats:italic> closely relates to radiotherapy (RT)\u2010related brain necrosis in nasopharyngeal carcinoma (NPC) patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Purpose<\/jats:title><jats:p>To explore RT\u2010related dynamic alterations in brain morphology and their potential genetic mechanism, and to explore the modulatory effects of <jats:italic>CEP128<\/jats:italic> genetic variants on RT\u2010related brain morphological alterations in NPC patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Study Type<\/jats:title><jats:p>Prospective, longitudinal.<\/jats:p><\/jats:sec><jats:sec><jats:title>Population<\/jats:title><jats:p>One hundred one patients with histopathologic ally\u2010proven NPC (age 41.64\u2009\u00b1\u20099.63, 46 male), analyzed at baseline (pre\u2010RT), 3\u2010months post\u2010RT and 6\u2009months post\u2010RT, and 19 sex\u2010, age\u2010 and education\u2010matched healthy controls.<\/jats:p><\/jats:sec><jats:sec><jats:title>Field Strength\/Sequence<\/jats:title><jats:p>3D gradient echo brain volume (3D\u2010BRAVO) and diffusion\u2010weighted single\u2010shot spin\u2010echo echo\u2010planar sequences at 3.0\u2009T.<\/jats:p><\/jats:sec><jats:sec><jats:title>Assessment<\/jats:title><jats:p>rs17111237 in <jats:italic>CEP<\/jats:italic>128 was detected by Sanger sequencing. Structural and diffusion images were processed with FreeSurfer and FSL. Morphometric similarity network (MSN) was constructed with nine cortical indices derived from structural and diffusion images.<\/jats:p><\/jats:sec><jats:sec><jats:title>Statistical Tests<\/jats:title><jats:p>One\u2010way ANOVA, chi\u2010square test. Pearson's correlation analysis was conducted to measure the relationship between <jats:italic>CEP128<\/jats:italic> gene\u2010expression level in human brain and MSN alterations. Repeated analysis of variance performed to assess group differences in MSN and the modulatory effects of the <jats:italic>CEP128<\/jats:italic> gene within patients. Significance level: <jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05, false\u2010discovery rate correction.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>RT\u2010related significant widespread MSN alterations were observed in the cortices of NPC patients. Notably, regional MSN alterations had a weak but significant negative correlation with the cortical pattern of <jats:italic>CEP128<\/jats:italic> gene expression (<jats:italic>r<\/jats:italic>\u2009=\u2009\u22120.152). Furthermore, rs17111237 in the <jats:italic>CEP128<\/jats:italic> had significant modulatory effects on the observed MSN alterations in NPC patients, with the modulatory effects being most obvious at 3\u2009months post\u2010RT.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>MSN has potential to serve as a sensitive biomarker to detect RT\u2010related brain injury. Inter\u2010brain regional and inter\u2010patient variability of RT\u2010related brain injuries may be attributed to the cortical expression of the <jats:italic>CEP128<\/jats:italic> gene and the modulatory effects of the promoter variant rs17111237 in <jats:italic>CEP128<\/jats:italic>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Evidence Level<\/jats:title><jats:p>2<\/jats:p><\/jats:sec><jats:sec><jats:title>Technical Efficacy<\/jats:title><jats:p>Stage 2<\/jats:p><\/jats:sec>","DOI":"10.1002\/jmri.28824","type":"journal-article","created":{"date-parts":[[2023,5,30]],"date-time":"2023-05-30T09:14:40Z","timestamp":1685438080000},"page":"648-658","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Modulation Effects of the <scp><i>CEP128<\/i><\/scp> Gene on Radiotherapy\u2010Related Brain Injury: A Longitudinal Structural Study Using Multi\u2010Parametric Brain <scp>MR<\/scp> Images"],"prefix":"10.1002","volume":"59","author":[{"given":"Shiwei","family":"Lin","sequence":"first","affiliation":[{"name":"Department of Radiology Huazhong University of Science and Technology Union Shenzhen Hospital  Shenzhen China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1712-630X","authenticated-orcid":false,"given":"Xiaofei","family":"Lv","sequence":"additional","affiliation":[{"name":"Department of Medical Imaging, Sun Yat\u2010sen University Cancer Center, State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine Guangdong Key Laboratory of Nasopharyngeal Carcinoma Diagnosis and Therapy  Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoshan","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Radiology Huazhong University of Science and Technology Union Shenzhen Hospital  Shenzhen 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