{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T04:06:32Z","timestamp":1785211592474,"version":"3.55.0"},"reference-count":40,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2023,10,5]],"date-time":"2023-10-05T00:00:00Z","timestamp":1696464000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003399","name":"Science and Technology Commission of Shanghai Municipality","doi-asserted-by":"publisher","award":["19411951200"],"award-info":[{"award-number":["19411951200"]}],"id":[{"id":"10.13039\/501100003399","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003399","name":"Science and Technology Commission of Shanghai Municipality","doi-asserted-by":"publisher","award":["19ZR1407900"],"award-info":[{"award-number":["19ZR1407900"]}],"id":[{"id":"10.13039\/501100003399","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Magnetic Resonance Imaging"],"published-print":{"date-parts":[[2024,7]]},"abstract":"Background<\/jats:title>Coupling between neuronal activity and blood perfusion is termed neurovascular coupling (NVC), and it provides a potentially new mechanistic perspective into understanding numerous brain diseases. Although abnormal brain activity and blood supply have been separately reported in mitochondrial myopathy, encephalopathy, lactic acidosis, and stroke\u2010like episodes (MELAS), whether anomalous NVC would be present is unclear.<\/jats:p><\/jats:sec>Purpose<\/jats:title>To investigate NVC changes and potential neural basis in MELAS by combining resting\u2010state functional MRI (rs\u2010fMRI) and arterial spin labeling (ASL).<\/jats:p><\/jats:sec>Study Type<\/jats:title>Prospective.<\/jats:p><\/jats:sec>Subjects<\/jats:title>Twenty\u2010four patients with MELAS (age: 29.8\u2009\u00b1\u20097.3\u2009years) in the acute stage and 24 healthy controls (HCs, age: 26.4\u2009\u00b1\u20098.1\u2009years). Additionally, 12 patients in the chronic stage were followed up.<\/jats:p><\/jats:sec>Field Strength\/Sequence<\/jats:title>3.0\u2009T, resting\u2010state gradient\u2010recalled echo\u2010planar imaging and pseudo\u2010continuous 3D ASL sequences.<\/jats:p><\/jats:sec>Assessment<\/jats:title>Amplitude of low\u2010frequency fluctuation (ALFF), fractional ALFF (fALFF), regional homogeneity (ReHo), and functional connectivity strength (FCS) were calculated from rs\u2010fMRI, and cerebral blood flow (CBF) was computed from ASL. Global NVC was assessed by correlation coefficients of CBF\u2010ALFF, CBF\u2010fALFF, CBF\u2010ReHo, and CBF\u2010FCS. Regional NVC was also evaluated by voxel\u2010wise and lesion\u2010wise ratios of CBF\/ALFF, CBF\/fALFF, CBF\/ReHo, and CBF\/FCS.<\/jats:p><\/jats:sec>Statistical Tests<\/jats:title>Two\u2010sample t<\/jats:italic>\u2010test, paired\u2010sample t<\/jats:italic>\u2010test, Gaussian random fields correction. A P<\/jats:italic> value <0.05 was considered statistically significant.<\/jats:p><\/jats:sec>Results<\/jats:title>Compared with HC, MELAS patients in acute stage showed significantly reduced global CBF\u2010ALFF, CBF\u2010fALFF, CBF\u2010ReHo, and CBF\u2010FCS coupling (P<\/jats:italic>\u2009<\u20090.001). Altered CBF\/ALFF, CBF\/fALFF, CBF\/ReHo, and CBF\/FCS ratios were found mainly distributed in the middle cerebral artery territory in MELAS patients. In addition, significantly increased NVC ratios were found in the acute stroke\u2010like lesions in acute stage (P<\/jats:italic>\u2009<\u20090.001), with a recovery trend in chronic stage.<\/jats:p><\/jats:sec>Data Conclusions<\/jats:title>This study showed dynamic alterations in NVC in MELAS patients from acute to chronic stage, which may provide a novel insight for understanding the pathogenesis of MELAS.<\/jats:p><\/jats:sec>Evidence Level<\/jats:title>2<\/jats:p><\/jats:sec>Technical Efficacy<\/jats:title>Stage 1<\/jats:p><\/jats:sec>","DOI":"10.1002\/jmri.29035","type":"journal-article","created":{"date-parts":[[2023,10,5]],"date-time":"2023-10-05T20:05:02Z","timestamp":1696536302000},"page":"327-336","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Altered Neurovascular Coupling in Patients With Mitochondrial Myopathy, Encephalopathy, Lactic Acidosis, and Stroke\u2010Like Episodes (MELAS<\/scp>): A Combined Resting\u2010State fMRI<\/scp> and Arterial Spin Labeling Study"],"prefix":"10.1002","volume":"60","author":[{"given":"Rong","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Radiology, Huashan Hospital Fudan University Shanghai China"},{"name":"Institute of Functional and Molecular Medical Imaging Fudan University Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1365-6813","authenticated-orcid":false,"given":"Xueling","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Radiology, Huashan Hospital Fudan University Shanghai China"},{"name":"Institute of Functional and Molecular Medical Imaging Fudan University Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chong","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Neurology, Huashan Hospital Fudan University Shanghai 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