{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T01:44:39Z","timestamp":1790646279269,"version":"4.1.0"},"reference-count":42,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2024,3,25]],"date-time":"2024-03-25T00:00:00Z","timestamp":1711324800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100005615","name":"Beth Israel Deaconess Medical Center","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100005615","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["AR057093"],"award-info":[{"award-number":["AR057093"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["K99\/R00"],"award-info":[{"award-number":["K99\/R00"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006823","name":"Boston Children's Hospital","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100006823","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Magnetic Resonance Imaging"],"published-print":{"date-parts":[[2024,12]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Osteoporosis (OP) and osteomalacia (OM) are metabolic bone diseases characterized by mineral and matrix density changes. Quantitative bone matrix density differentiates OM from OP. MRI is a noninvasive and nonionizing imaging technique that can measure bone matrix density quantitatively in ex vivo and in vivo.<\/jats:p><\/jats:sec><jats:sec><jats:title>Purpose<\/jats:title><jats:p>To demonstrate water\u2009+\u2009fat suppressed <jats:sup>1<\/jats:sup>H MRI to compute bone matrix density in ex vivo rat femurs in the preclinical model.<\/jats:p><\/jats:sec><jats:sec><jats:title>Study Type<\/jats:title><jats:p>Prospective.<\/jats:p><\/jats:sec><jats:sec><jats:title>Animal Model<\/jats:title><jats:p>Fifteen skeletally mature female Sprague\u2013Dawley rats, five per group (normal, ovariectomized (OVX), partially nephrectomized\/vitamin D (Vit\u2010D) deficient), 250\u2013275\u2009g, \u223c15\u2009weeks old.<\/jats:p><\/jats:sec><jats:sec><jats:title>Field Strength\/Sequence<\/jats:title><jats:p>7T, zero echo time sequence with water\u2009+\u2009fat (VAPOR) suppression capability, <jats:bold>\u03bc<\/jats:bold>CT imaging, and gravimetric measurements.<\/jats:p><\/jats:sec><jats:sec><jats:title>Assessment<\/jats:title><jats:p>Cortical and trabecular bone segments from normal and disease models were scanned in the same coil along with a dual calibration phantom for quantitative assessment of bone matrix density.<\/jats:p><\/jats:sec><jats:sec><jats:title>Statistical Tests<\/jats:title><jats:p>ANOVA and linear regression were used for data analysis, with <jats:italic>P<\/jats:italic>\u2010values &lt;0.05 statistically significant.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The MRI\u2010derived three\u2010density PEG pellet densities have a strong linear relationship with physical density measures (<jats:italic>r<\/jats:italic><jats:sup>2<\/jats:sup>\u2009=\u20090.99). The Vit\u2010D group had the lowest bone matrix density for cortical bone (0.47\u2009\u00b1\u20090.16\u2009g\u2009cm<jats:sup>\u22123<\/jats:sup>), whereas the OVX had the lowest bone matrix density for trabecular bone (0.26\u2009\u00b1\u20090.04\u2009g\u2009cm<jats:sup>\u22123<\/jats:sup>). Gravimetry results confirmed these MRI\u2010based observations for Vit\u2010D cortical (0.51\u2009\u00b1\u20090.07\u2009g\u2009cm<jats:sup>\u22123<\/jats:sup>) and OVX trabecular (0.26\u2009\u00b1 0.03\u2009g\u2009cm<jats:sup>\u22123<\/jats:sup>) bone groups.<\/jats:p><\/jats:sec><jats:sec><jats:title>Data Conclusion<\/jats:title><jats:p>Rat femur images were obtained using a modified pulse sequence and a custom\u2010designed double\u2010tuned (<jats:sup>1<\/jats:sup>H\/<jats:sup>31<\/jats:sup>P) transmit\u2010receive solenoid\u2010coil on a 7T preclinical MRI scanner. Phantom experiments confirmed a strong linear relation between MRI\u2010derived and physical density measures and quantitative bone matrix densities in rat femurs from normal, OVX, and Vit\u2010D deficient\/partially nephrectomized animals were computed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Level of Evidence<\/jats:title><jats:p>2<\/jats:p><\/jats:sec><jats:sec><jats:title>Technical Efficacy<\/jats:title><jats:p>Stage 2<\/jats:p><\/jats:sec>","DOI":"10.1002\/jmri.29361","type":"journal-article","created":{"date-parts":[[2024,3,25]],"date-time":"2024-03-25T12:41:09Z","timestamp":1711370469000},"page":"2423-2432","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["<scp>Q<\/scp>uantitative <sup>1<\/sup>H Magnetic Resonance Imaging on Normal and Pathologic Rat Bones by Solid\u2010State <sup>1<\/sup>H ZTE Sequence with Water and Fat Suppression"],"prefix":"10.1002","volume":"60","author":[{"given":"V\u00edctor B.","family":"Kassey","sequence":"first","affiliation":[{"name":"Musculoskeletal Translational Innovation Initiative, Carl J. Shapiro Department of Orthopaedic Surgery, Beth Israel Deaconess Medical Center  Boston Massachusetts USA"},{"name":"Department of Orthopaedic Surgery, Children's Hospital  Boston Massachusetts USA"},{"name":"Athinoula Martinos Center for Biomedical Imaging, Department of Radiology, Massachusetts General Hospital  Charlestown Massachusetts USA"},{"name":"Harvard Medical School  Boston Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthias","family":"Walle","sequence":"additional","affiliation":[{"name":"Musculoskeletal Translational Innovation Initiative, Carl J. Shapiro Department of Orthopaedic Surgery, Beth Israel Deaconess Medical Center  Boston Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonathan","family":"Egan","sequence":"additional","affiliation":[{"name":"Musculoskeletal Translational Innovation Initiative, Carl J. Shapiro Department of Orthopaedic Surgery, Beth Israel Deaconess Medical Center  Boston Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diana","family":"Yeritsyan","sequence":"additional","affiliation":[{"name":"Musculoskeletal Translational Innovation Initiative, Carl J. Shapiro Department of Orthopaedic Surgery, Beth Israel Deaconess Medical Center  Boston Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Indeevar","family":"Beeram","sequence":"additional","affiliation":[{"name":"Musculoskeletal Translational Innovation Initiative, Carl J. 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