{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,17]],"date-time":"2025-10-17T00:23:38Z","timestamp":1760660618751,"version":"build-2065373602"},"reference-count":11,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2006,6,29]],"date-time":"2006-06-29T00:00:00Z","timestamp":1151539200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Mass Spectrom."],"published-print":{"date-parts":[[2006,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We have developed and validated a simple detection system with high\u2010performance liquid chromatography (HPLC) with positive ion electrospray ionization tandem mass spectrometry (ESI\u2010MS\/MS) for determining cimetropium levels in human plasma using scopolamine butyl bromide as an internal standard (I.S.). The acquisition was performed in the multiple reaction monitoring (MRM) mode, by monitoring the transitions: <jats:italic>m<\/jats:italic>\/<jats:italic>z<\/jats:italic> 357.9 &gt; 103.1 for cimetropium and <jats:italic>m<\/jats:italic>\/<jats:italic>z<\/jats:italic> 359.9 &gt; 103.1 for butyl\u2010scopolamine. The method involves a simple single\u2010step liquid\u2013liquid extraction with dichloromethane. The analyte was chromatographed on an YMC C18 reversed\u2010phase chromatographic column by isocratic elution with 10 m<jats:sc>M<\/jats:sc> ammonium formate buffer\u2013methanol (19 : 81, v\/v; adjusted to pH 4.0 with formic acid). The results were linear over the studied range (0.2\u2013100 ng ml<jats:sup>\u22121<\/jats:sup>), with <jats:italic>r<\/jats:italic><jats:sup>2<\/jats:sup> = 1.0000, and the total analysis time for each run was 2 min. Intra\u2010 and interassay precisions were 0.70\u20138.54% and 1.08\u20134.85%, respectively, and intra\u2010 and interassay accuracies were 97.56\u2013108.23% and 97.48\u2013103.91%, respectively. The lower limit of quantification (LLOQ) was 0.2 ng ml<jats:sup>\u22121<\/jats:sup>. At this concentration, mean intra\u2010 and interassay precisions were 8.54% and 4.85%, respectively, and mean intra\u2010 and interassay accuracies were 97.56% and 98.91%, respectively. The mean recovery ranged from 62.71 \u00b1 4.06 to 64.23 \u00b1 2.32%. Cimetropium was found to be stable in plasma samples under typical storage and processing conditions. The devised assay was successfully applied to a pharmacokinetic study of cimetropium bromide administered as a single oral dose (150 mg) to healthy volunteers. Copyright \u00a9 2006 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/jms.1043","type":"journal-article","created":{"date-parts":[[2006,6,30]],"date-time":"2006-06-30T06:46:34Z","timestamp":1151649994000},"page":"855-860","source":"Crossref","is-referenced-by-count":4,"title":["A rapid and sensitive liquid chromatography\/positive ion tandem mass spectrometry method for the determination of cimetropium in human plasma by liquid\u2013liquid extraction"],"prefix":"10.1002","volume":"41","author":[{"given":"Heon\u2010Woo","family":"Lee","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wan\u2010Su","family":"Park","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yong\u2010Won","family":"Kim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sung\u2010Hee","family":"Cho","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sung\u2010Su","family":"Kim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ji\u2010Hyung","family":"Seo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kyung\u2010Tae","family":"Lee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,6,29]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM196306272682608"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/0016-5085(81)90275-4"},{"key":"e_1_2_6_4_2","first-page":"796","article-title":"Cimetropium: characterization of antimuscarinic and spasmolytic properties","volume":"35","author":"Schiavone A","year":"1985","journal-title":"Arzneim.\u2010Forsch."},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1159\/000199839"},{"key":"e_1_2_6_6_2","first-page":"1262","article-title":"Oral cimetropium bromide, a new antimuscarinic drug, for long\u2010term treatment of irritable bowel syndrome","volume":"83","author":"Centonze V","year":"1988","journal-title":"Am. 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