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Sample preparation includes a simple and fast deproteinization with acetonitrile and a back\u2010extraction of the acetonitrile with dichloromethane. Chromatography is performed on a reversed\u2010phase PLRP\u2010S polymeric column using 0.05% formic acid in water and acetonitrile. The limit of quantification is 25 ng\/ml, which is lower than other published methods using ultraviolet (UV), fluorimetric or mass spectrometric detection. The limit of detection is calculated to be 3.5 ng\/ml. The stability of clavulanic acid was demonstrated according to The Guidelines of Bioanalytical Method Validation of The Food and Drug Administration (FDA): freeze and thaw stability, short\u2010term stability, long\u2010term stability, stock solution stability and postpreparative stability. The method is used in a pharmacokinetic and bioequivalence study of amoxycillin\/clavulanic acid formulations in calves. Copyright \u00a9 2006 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/jms.1106","type":"journal-article","created":{"date-parts":[[2006,10,18]],"date-time":"2006-10-18T10:51:32Z","timestamp":1161168692000},"page":"1414-1420","source":"Crossref","is-referenced-by-count":22,"title":["Determination of clavulanic acid in calf plasma by liquid chromatography tandem mass spectrometry"],"prefix":"10.1002","volume":"41","author":[{"given":"Tim","family":"Reyns","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siegrid","family":"De Baere","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siska","family":"Croubels","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick","family":"De Backer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,10,18]]},"reference":[{"issue":"1","key":"e_1_2_6_2_2","doi-asserted-by":"crossref","first-page":"i3","DOI":"10.1093\/jac\/dkh050","article-title":"Augmentin\u00ae (amoxicillin\/clavulanate) in the treatment of community\u2010acquired respiratory tract infection: a review of the continuing development of an innovative antimicrobial agent","volume":"53","author":"White R","year":"2004","journal-title":"J. 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