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Although the decarboxylation of \u03949\u2010THCA\u2010A to \u03949\u2010THC was assumed to be complete\u2014which means that no \u03949\u2010THCA\u2010A should be detectable in urine and blood serum of cannabis consumers\u2014we found \u03949\u2010THCA\u2010A in the urine and blood serum samples collected from police controls of drivers suspected for driving under the influence of drugs (DUID). For LC\u2010MS\/MS analysis, urine and blood serum samples were prepared by solid\u2010phase extraction. Analysis was performed with a phenylhexyl column using gradient elution with acetonitrile. For detection of \u03949\u2010THCA\u2010A, the mass spectrometer (MS) (SCIEX API 365 triple\u2010quadrupole MS with TurboIonSpray source) was operated in the multiple reaction monitoring (MRM) mode using the following transitions: <jats:italic>m<\/jats:italic>\/<jats:italic>z<\/jats:italic>357 \u2192 313, <jats:italic>m<\/jats:italic>\/<jats:italic>z<\/jats:italic>357 \u2192 245 and <jats:italic>m<\/jats:italic>\/<jats:italic>z<\/jats:italic>357 \u2192 191. \u03949\u2010THCA\u2010A could be detected in the urine and blood serum samples of several cannabis consumers in concentrations of up to 10.8 ng\/ml in urine and 14.8 ng\/ml in serum. The concentration of \u03949\u2010THCA\u2010A was below the \u03949\u2010THC concentration in most serum samples, resulting in molar ratios of \u03949\u2010THCA\u2010A\/\u03949\u2010THC of approximately 5.0\u201318.6%. Only in one case, where a short elapsed time between the last intake and blood sampling is assumed, the molar ratio was 18.6% in the serum. This indicates differences in elimination kinetics, which need to be investigated in detail. 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