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We present here imaging mass spectrometry results obtained with a high\u2010resolution scanning MALDI time\u2010of\u2010flight mass spectrometer, equipped with a coaxial laser illumination ion source, capable of achieving irradiation areas as small as 40 \u00b5m<jats:sup>2<\/jats:sup> (<jats:italic>ca<\/jats:italic> 7 \u00b5m diameter). MALDI\u2010generated analyte ion signals from these very small irradiation volumes can be observed in a molecular weight range up to 27 000. High\u2010resolution imaging mass spectrometry images were successfully generated from matrix thin film samples and tissue sections with scanning resolutions at and below 10 \u00b5m. This work also provides fundamental characterization of the ion signal dependence as a function of various focus and fluence parameters that will be required for extension to tissue imaging at the subcellular level. Copyright \u00a9 2007 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/jms.1180","type":"journal-article","created":{"date-parts":[[2007,2,28]],"date-time":"2007-02-28T07:32:22Z","timestamp":1172647942000},"page":"476-489","source":"Crossref","is-referenced-by-count":127,"title":["Instrument design and characterization for high resolution MALDI\u2010MS imaging of tissue sections"],"prefix":"10.1002","volume":"42","author":[{"given":"Pierre","family":"Chaurand","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenneth E.","family":"Schriver","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard M.","family":"Caprioli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2007,2,28]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1021\/cr980137w"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/jms.153"},{"key":"e_1_2_6_4_2","doi-asserted-by":"crossref","first-page":"86A","DOI":"10.1021\/ac0415197","article-title":"Profiling and imaging proteins in tissue sections by mass spectrometry","volume":"76","author":"Chaurand P","year":"2004","journal-title":"Anal. 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