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Some of these phospholipid oxidation products have potent biological activities and likely contribute to human pathophysiological conditions. Oxidation products have also been used as markers of oxidative stress <jats:italic>in vivo<\/jats:italic>. Identification and quantification of phospholipid oxidation products are often performed by analyzing the oxidized free fatty acid moieties after hydrolysis from the phospholipids head groups by gas chromatography\u2013mass spectrometry (GC\u2013MS) or liquid chromatography\u2013mass spectrometry (LC\u2013MS). We now describe the definitive identification of intact oxidized products of glycerophospholipids including glycerophosphatidylcholine (GPC), glycerophosphatidylethanolamine (GPE), and glycerophosphatidylserine (GPS) <jats:italic>in vitro<\/jats:italic> and <jats:italic>in vivo<\/jats:italic> using iontrap MS. For these analyses, the negative ions of the oxidation products of phospholipids are fragmented to MS<jats:sup><jats:italic>n<\/jats:italic><\/jats:sup> and unequivocal structural characterization is obtained based on collision\u2010induced dissociation (CID) of the <jats:italic>sn<\/jats:italic>\u20102 carboxylate ion. This technique overcomes the need to hydrolyze fatty acids from phospholipids in the analysis. The method has been used to identify a number of oxidation products of glycerophospholipids including hydroxyeicosatetraenoates (HETEs) and isoprostanes (IsoPs) esterified to different classes of glycerophospholipids <jats:italic>in vitro<\/jats:italic> and <jats:italic>in vivo<\/jats:italic>. These studies thus provide a new approach to identify the intact oxidation products of glycerolphospholipids. Copyright \u00a9 2009 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/jms.1542","type":"journal-article","created":{"date-parts":[[2009,5,11]],"date-time":"2009-05-11T05:59:43Z","timestamp":1242021583000},"page":"672-680","source":"Crossref","is-referenced-by-count":42,"title":["Identification of intact oxidation products of glycerophospholipids <i>in vitro<\/i> and <i>in vivo<\/i> using negative ion electrospray iontrap mass spectrometry"],"prefix":"10.1002","volume":"44","author":[{"given":"Huiyong","family":"Yin","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brian E.","family":"Cox","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ned A.","family":"Porter","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jason D.","family":"Morrow","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ginger L.","family":"Milne","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2009,1,5]]},"reference":[{"key":"e_1_2_5_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)62347-4"},{"key":"e_1_2_5_3_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.ATV.0000152605.64964.c0"},{"key":"e_1_2_5_4_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMp058118"},{"key":"e_1_2_5_5_2","first-page":"915","article-title":"Beyond cholesterol\u2014modifications of low\u2010density lipoprotein that increase its atherogenicity","volume":"320","author":"Steinberg D.","year":"1989","journal-title":"The New England Journal of Medicine"},{"key":"e_1_2_5_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbadis.2007.04.009"},{"key":"e_1_2_5_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/mas.10061"},{"key":"e_1_2_5_8_2","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1016\/j.ymeth.2006.05.014","article-title":"Lipidomics: an analysis of cellular lipids by ESI\u2010MS","volume":"39","author":"Milne S.","year":"2006","journal-title":"Methods: Analytical Methods in the Science of Lipidomics, Membrane Organization and Protein\u2013Lipid Interactions"},{"key":"e_1_2_5_9_2","doi-asserted-by":"publisher","DOI":"10.1194\/jlr.D500010-JLR200"},{"key":"e_1_2_5_10_2","doi-asserted-by":"publisher","DOI":"10.1194\/jlr.R600022-JLR200"},{"key":"e_1_2_5_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/0891-5849(95)02173-6"},{"key":"e_1_2_5_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1050-1738(01)00098-6"},{"key":"e_1_2_5_13_2","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2005.7.170"},{"key":"e_1_2_5_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm1626"},{"key":"e_1_2_5_15_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.87.23.9383"},{"key":"e_1_2_5_16_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M300604200"},{"key":"e_1_2_5_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1044-0305(98)00013-0"},{"key":"e_1_2_5_18_2","doi-asserted-by":"publisher","DOI":"10.1006\/abio.1998.2602"},{"key":"e_1_2_5_19_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11745-001-0841-2"},{"key":"e_1_2_5_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2004.09.017"},{"key":"e_1_2_5_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(99)00106-8"},{"key":"e_1_2_5_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2005.03.038"},{"key":"e_1_2_5_23_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M303984200"},{"key":"e_1_2_5_24_2","doi-asserted-by":"publisher","DOI":"10.1194\/jlr.R400001-JLR200"},{"key":"e_1_2_5_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2008.02.043"},{"key":"e_1_2_5_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chemphyslip.2008.07.003"},{"key":"e_1_2_5_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ab.2005.04.042"},{"key":"e_1_2_5_28_2","doi-asserted-by":"publisher","DOI":"10.1002\/ana.21168"},{"key":"e_1_2_5_29_2","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.520"},{"key":"e_1_2_5_30_2","doi-asserted-by":"publisher","DOI":"10.1021\/cr9900883"},{"key":"e_1_2_5_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4347(99)00207-8"},{"key":"e_1_2_5_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/0090-6980(94)90011-6"},{"key":"e_1_2_5_33_2","doi-asserted-by":"crossref","first-page":"4317","DOI":"10.1016\/S0021-9258(17)41781-9","article-title":"Free radical\u2010induced generation of isoprostanes in vivo. 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