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This work involved the development of a continuous affinity capture and concentration system, providing cost\u2010effective and structural analysis of drug candidates in a flow\u2010through format. The system combines the strengths of a hollow fiber dialysis membrane of ease and speed of purification and concentration with the specificity of affinity interactions in solution. The complexes between the lead compounds and the affinity binding proteins are separated from other chemical components inside a dialysis hollow fiber as the result of their differences in size. The affinity complexes are further concentrated inside a second dialysis fiber. The concentrated drug candidates are liberated from the binding proteins in a microdialysis junction and can be directly identified using electrospray ionization mass spectrometry. Two model systems, including human serum albumin\u2013warfarin\u2010related compounds and anti\u2010phenobarbital antibody\u2013barbiturates, were employed for mechanistic studies of dialysis versus dissociation kinetics and competitive selection of drug candidates according to their binding strengths. Copyright \u00a9 2001 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/jms.168","type":"journal-article","created":{"date-parts":[[2002,8,25]],"date-time":"2002-08-25T17:57:50Z","timestamp":1030298270000},"page":"664-669","source":"Crossref","is-referenced-by-count":16,"title":["On\u2010line coupling of hollow fiber membranes with electrospray ionization mass spectrometry for continuous affinity selection, concentration and identification of small\u2010molecule libraries"],"prefix":"10.1002","volume":"36","author":[{"given":"Yun","family":"Jiang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cheng S.","family":"Lee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2001,6,21]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/anie.199203673"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.15.6909"},{"key":"e_1_2_6_4_2","doi-asserted-by":"crossref","first-page":"22 129","DOI":"10.1016\/S0021-9258(17)31765-9","volume":"269","author":"Dennus MS","year":"1994","journal-title":"J. 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