{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T14:14:32Z","timestamp":1789913672077,"version":"4.0.1"},"reference-count":16,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2010,4,13]],"date-time":"2010-04-13T00:00:00Z","timestamp":1271116800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Mass Spectrom."],"published-print":{"date-parts":[[2010,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The group of biologically important amines includes putrescine, spermidine and spermine, as well as agmatine, which is a guanidino\u2010amine. There is considerable evidence supporting a role of these amines in the etiology and pathology of mental disorders. We have previously developed a quantitative GC\u2010MS method for simultaneous measurement of three major polyamines to support our studies linking polyamines to mental disorders. However, a unique GC\u2010MS method is required for agmatine. To efficiently extract agmatine from postmortem brain tissues, we developed an isopropanol based liquid\u2013liquid extraction protocol using potassium carbonate as a salting\u2010out agent which showed a much greater recovery than n\u2010butanol used in earlier methods. The GC\u2010MS analysis employed hexafluoroacetylacetone as derivatization reagent and was carried out using negative chemical ionization with total ion and selected ion monitoring. <jats:sup>15<\/jats:sup>N<jats:sub>4<\/jats:sub>\u2010Agmatine was synthesized from <jats:sup>15<\/jats:sup>N<jats:sub>4<\/jats:sub>\u2010<jats:sc>L<\/jats:sc>\u2010arginine and used as internal standard in a conventional stable isotope dilution assay. This method accurately measures the level of agmatine from very small quantities (10\u201320 mg) of postmortem brain tissue, with a quantitation limit down to 1 ng\/g of wet tissue. The limit of detection is 0.01 ng\/g of wet tissue. Copyright \u00a9 2010 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/jms.1742","type":"journal-article","created":{"date-parts":[[2010,4,13]],"date-time":"2010-04-13T14:08:51Z","timestamp":1271167731000},"page":"560-565","source":"Crossref","is-referenced-by-count":6,"title":["A novel liquid\u2013liquid extraction and stable isotope dilution NCI\u2010GC\u2010MS method for quantitation of agmatine in postmortem brain cortex"],"prefix":"10.1002","volume":"45","author":[{"given":"Gary Gang","family":"Chen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gustavo","family":"Turecki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Orval A.","family":"Mamer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2010,4,13]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1139\/jpn.0812","article-title":"Implication of the polyamine system in mental disorders","volume":"33","author":"Fiori L. 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