{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T22:58:44Z","timestamp":1788217124252,"version":"build-2803163510"},"reference-count":27,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2010,7,7]],"date-time":"2010-07-07T00:00:00Z","timestamp":1278460800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Mass Spectrom."],"published-print":{"date-parts":[[2010,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The fragmentation behavior of a novel thiourea\u2010based cross\u2010linker molecule specifically designed for collision\u2010induced dissociation (CID) MS\/MS experiments is described. The development of this cross\u2010linker is part of our ongoing efforts to synthesize novel reagents, which create either characteristic fragment ions or indicative constant neutral losses (CNLs) during tandem mass spectrometry allowing a selective and sensitive analysis of cross\u2010linked products. The new derivatizing reagent for chemical cross\u2010linking solely contains a thiourea moiety that is flanked by two amine\u2010reactive <jats:italic>N<\/jats:italic>\u2010hydroxy succinimide (NHS) ester moieties for reaction with lysines or free <jats:italic>N<\/jats:italic>\u2010termini in proteins. The new reagent offers simple synthetic access and easy structural variation of either length or functionalities at both ends. The thiourea moiety exhibits specifically tailored CID fragmentation capabilities\u2014a characteristic CNL of 85 u\u2014ensuring a reliable detection of derivatized peptides by both electrospray ionization (ESI) and matrix\u2010assisted laser desorption\/ionization (MALDI) tandem mass spectrometry and as such possesses a versatile applicability for chemical cross\u2010linking studies. A detailed examination of the CID behavior of the presented thiourea\u2010based reagent reveals that slight structural variations of the reagent will be necessary to ensure its comprehensive and efficient application for chemical cross\u2010linking of proteins. Copyright \u00a9 2010 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/jms.1775","type":"journal-article","created":{"date-parts":[[2010,7,7]],"date-time":"2010-07-07T10:43:55Z","timestamp":1278499435000},"page":"880-891","source":"Crossref","is-referenced-by-count":25,"title":["Fragmentation behavior of a thiourea\u2010based reagent for protein structure analysis by collision\u2010induced dissociative chemical cross\u2010linking"],"prefix":"10.1002","volume":"45","author":[{"given":"Mathias Q.","family":"M\u00fcller","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frank","family":"Dreiocker","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian H.","family":"Ihling","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mathias","family":"Sch\u00e4fer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"Sinz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2010,7,7]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/jms.559"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1021\/bc050043a"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/mas.20082"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.090099097"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1044-0305(00)00212-9"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac0613840"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.3213"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.T400016-MCP200"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/jms.1702"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.2675315"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac00171a028"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pep.2008.07.016"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/0040-4020(93)80004-D"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1002\/cber.19761090910"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1055\/s-2003-41435"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1021\/jo052576i"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja972497r"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0040-4039(02)01759-8"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0039-128X(00)00091-X"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1002\/prot.21445"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0968-0004(01)01954-5"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1742-4658.2005.05053.x"},{"key":"e_1_2_6_24_2","unstructured":"http:\/\/www2.lib.udel.edu\/database\/intermed\/scifind\/viewer.html; Accelrys ViewerLite 5.0 Software."},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1044-0305(03)00327-1"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-008-2231-5"},{"key":"e_1_2_6_27_2","doi-asserted-by":"publisher","DOI":"10.1002\/jms.1544"},{"key":"e_1_2_6_28_2","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.1144"}],"container-title":["Journal of Mass Spectrometry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjms.1775","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jms.1775","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T13:39:05Z","timestamp":1760621945000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/jms.1775"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,7,7]]},"references-count":27,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2010,8]]}},"alternative-id":["10.1002\/jms.1775"],"URL":"https:\/\/doi.org\/10.1002\/jms.1775","archive":["Portico"],"relation":{},"ISSN":["1076-5174","1096-9888"],"issn-type":[{"value":"1076-5174","type":"print"},{"value":"1096-9888","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,7,7]]}}}