{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,7]],"date-time":"2025-11-07T18:59:22Z","timestamp":1762541962889,"version":"build-2065373602"},"reference-count":30,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2010,10,21]],"date-time":"2010-10-21T00:00:00Z","timestamp":1287619200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Mass Spectrom."],"published-print":{"date-parts":[[2010,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A simple, rapid, straightforward and washing\/separation free of in\u2010solution digestion method for microwave\u2010assisted tryptic digestion of proteins (cytochrome <jats:italic>c<\/jats:italic>, lysozyme and myoglobin) using bare TiO<jats:sub>2<\/jats:sub> nanoparticles (NPs) prepared in aqueous solution to serve as multifunctional nanoprobes in electrospray ionization mass spectrometry (ESI\u2010MS) was demonstrated. The current approach is termed as \u2018on particle ionization\/enrichment (OPIE)\u2019 and it can be applied in ESI\u2010MS, atmospheric pressure\u2010matrix\u2010assisted laser desorption\/ionization mass spectrometry (AP\u2010MALDI\u2010MS) and matrix\u2010assisted laser desorption\/ionization time\u2010of\u2010flight mass spectrometry (MALDI\u2010TOF\u2010MS). The bare TiO<jats:sub>2<\/jats:sub> NPs can assist, accelerate and effectively enhance the digestion efficiency, sequence coverage and detection sensitivity of peptides for the microwave\u2010assisted tryptic digestion of proteins in ESI\u2010MS. The reason is attributed to the fact that proteins or partially digested proteins are easily attracted or concentrated onto the surface of TiO<jats:sub>2<\/jats:sub> NPs, resulting in higher efficiency of digestion reactions in the microwave experiments. Besides, the TiO<jats:sub>2<\/jats:sub> NPs could act as a microwave absorber to accelerate and enrich the protein fragments in a short period of time (40\u201360 s) from the microwave experiments in ESI\u2010MS. Furthermore, the bare TiO<jats:sub>2<\/jats:sub> NPs prepared in aqueous solution exhibit high adsorption capability toward the protein fragments (peptides); thus, the OPIE approach for detecting the digested protein fragments via ESI and MALDI ionization could be achieved. The current technique is also a washing and separation\u2010free technique for accelerating and enriching microwave\u2010assisted tryptic digestion of proteins in the ESI\u2010MS and MALDI\u2010MS. It exhibits potential to be widely applied to biotechnology and proteome research in the near future. Copyright \u00a9 2010 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/jms.1855","type":"journal-article","created":{"date-parts":[[2010,10,21]],"date-time":"2010-10-21T07:19:59Z","timestamp":1287645599000},"page":"1402-1408","source":"Crossref","is-referenced-by-count":7,"title":["On particle ionization\/enrichment of multifunctional nanoprobes: washing\/separation\u2010free, acceleration and enrichment of microwave\u2010assisted tryptic digestion of proteins via bare TiO<sub>2<\/sub> nanoparticles in ESI\u2010MS and comparing to MALDI\u2010MS"],"prefix":"10.1002","volume":"45","author":[{"given":"Hui\u2010Fen","family":"Wu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kavita","family":"Agrawal","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kamlesh","family":"Shrivas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yi\u2010Hsien","family":"Lee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2010,10,21]]},"reference":[{"doi-asserted-by":"publisher","key":"e_1_2_7_2_2","DOI":"10.1002\/pmic.200700617"},{"doi-asserted-by":"publisher","key":"e_1_2_7_3_2","DOI":"10.1021\/ac000176j"},{"doi-asserted-by":"publisher","key":"e_1_2_7_4_2","DOI":"10.1002\/pmic.200500234"},{"doi-asserted-by":"publisher","key":"e_1_2_7_5_2","DOI":"10.1039\/b600752j"},{"doi-asserted-by":"publisher","key":"e_1_2_7_6_2","DOI":"10.1016\/j.trac.2006.05.015"},{"doi-asserted-by":"publisher","key":"e_1_2_7_7_2","DOI":"10.1110\/ps.0213702"},{"doi-asserted-by":"publisher","key":"e_1_2_7_8_2","DOI":"10.1016\/j.jasms.2005.01.012"},{"doi-asserted-by":"publisher","key":"e_1_2_7_9_2","DOI":"10.1002\/pmic.200401056"},{"doi-asserted-by":"publisher","key":"e_1_2_7_10_2","DOI":"10.1021\/ac0614893"},{"doi-asserted-by":"publisher","key":"e_1_2_7_11_2","DOI":"10.1016\/j.chroma.2008.03.043"},{"doi-asserted-by":"publisher","key":"e_1_2_7_12_2","DOI":"10.1021\/la026252q"},{"doi-asserted-by":"publisher","key":"e_1_2_7_13_2","DOI":"10.1016\/j.apcatb.2007.01.016"},{"doi-asserted-by":"publisher","key":"e_1_2_7_14_2","DOI":"10.1002\/cbic.200300707"},{"doi-asserted-by":"publisher","key":"e_1_2_7_15_2","DOI":"10.1016\/j.bioelechem.2004.03.008"},{"doi-asserted-by":"publisher","key":"e_1_2_7_16_2","DOI":"10.1016\/j.bioelechem.2005.10.003"},{"doi-asserted-by":"publisher","key":"e_1_2_7_17_2","DOI":"10.1016\/S0891-5849(97)00068-3"},{"doi-asserted-by":"publisher","key":"e_1_2_7_18_2","DOI":"10.1016\/S1010-6030(00)00389-0"},{"doi-asserted-by":"publisher","key":"e_1_2_7_19_2","DOI":"10.1002\/rcm.3058"},{"doi-asserted-by":"publisher","key":"e_1_2_7_20_2","DOI":"10.1021\/la700555y"},{"doi-asserted-by":"publisher","key":"e_1_2_7_21_2","DOI":"10.1016\/j.matlet.2005.12.100"},{"doi-asserted-by":"publisher","key":"e_1_2_7_22_2","DOI":"10.1021\/ac034986h"},{"doi-asserted-by":"publisher","key":"e_1_2_7_23_2","DOI":"10.1021\/ac050831t"},{"doi-asserted-by":"publisher","key":"e_1_2_7_24_2","DOI":"10.1021\/ac060833t"},{"doi-asserted-by":"publisher","key":"e_1_2_7_25_2","DOI":"10.1039\/B716055K"},{"doi-asserted-by":"publisher","key":"e_1_2_7_26_2","DOI":"10.1002\/pmic.200800772"},{"doi-asserted-by":"publisher","key":"e_1_2_7_27_2","DOI":"10.1016\/j.compscitech.2006.01.001"},{"doi-asserted-by":"publisher","key":"e_1_2_7_28_2","DOI":"10.1002\/rcm.4273"},{"doi-asserted-by":"publisher","key":"e_1_2_7_29_2","DOI":"10.1007\/s00216-010-3573-3"},{"doi-asserted-by":"publisher","key":"e_1_2_7_30_2","DOI":"10.1016\/j.colsurfb.2008.09.014"},{"doi-asserted-by":"publisher","key":"e_1_2_7_31_2","DOI":"10.1021\/ac051162m"}],"container-title":["Journal of Mass Spectrometry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjms.1855","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jms.1855","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T14:42:31Z","timestamp":1760625751000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/jms.1855"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,10,21]]},"references-count":30,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2010,12]]}},"alternative-id":["10.1002\/jms.1855"],"URL":"https:\/\/doi.org\/10.1002\/jms.1855","archive":["Portico"],"relation":{},"ISSN":["1076-5174","1096-9888"],"issn-type":[{"type":"print","value":"1076-5174"},{"type":"electronic","value":"1096-9888"}],"subject":[],"published":{"date-parts":[[2010,10,21]]}}}