{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T16:31:43Z","timestamp":1762101103300,"version":"build-2065373602"},"reference-count":42,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2010,11,4]],"date-time":"2010-11-04T00:00:00Z","timestamp":1288828800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Mass Spectrom."],"published-print":{"date-parts":[[2010,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>For the first time, we utilized multifunctional nanoparticles composite (NPs composite) for matrix\u2010assisted laser desorption\/ionization mass spectrometric (MALDI\u2010MS) analysis of peptides and proteins. Multiwalled carbon nanotubes doped with Cd<jats:sup>2+<\/jats:sup> ions and modified with cadmium sulfide NPs were synthesized by a chemical reduction method at room temperature. The multifunctional NPs composite applied for the analysis of peptides and microwave\u2010digested proteins in the atmospheric pressure matrix\u2010assisted laser desorption\/ionization ion\u2010trap and MALDI time\u2010of\u2010flight (TOF) mass spectrometry (MS) was successfully demonstrated. The maximum detection sensitivity for peptides in MALDI\u2010MS was achieved by the adsorption of negatively charged peptides onto the surfaces of NP composite through electrostatic interactions. The optimal conditions of peptide mixtures were obtained at 20 min of incubation time using 1 mg of NPs composite when the pH of the sample solution was kept higher than the p<jats:italic>I<\/jats:italic> values of peptides. The potentiality of the NP composite in the preconcentration of peptides was compared with that of the individual NP by calculating the preconcentration factors (PF) and found that the NPs composite showed a 4\u20136 times of PF than the other NPs. In addition, the NPs composite was also applied as heat\u2010absorbing materials for efficient microwave tryptic digestion of cytochrome <jats:italic>c<\/jats:italic> and lysozyme from milk protein in MALDI\u2010TOF\u2010MS analysis. We believe that the use of NPs composite technique would be an efficient and powerful preconcentrating tool for MALDI\u2010MS for the study of proteome research. Copyright \u00a9 2010 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/jms.1861","type":"journal-article","created":{"date-parts":[[2010,11,6]],"date-time":"2010-11-06T00:07:11Z","timestamp":1289002031000},"page":"1452-1460","source":"Crossref","is-referenced-by-count":68,"title":["Multifunctional nanoparticles composite for MALDI\u2010MS: Cd<sup>2+<\/sup>\u2010doped carbon nanotubes with CdS nanoparticles as the matrix, preconcentrating and accelerating probes of microwave enzymatic digestion of peptides and proteins for direct MALDI\u2010MS analysis"],"prefix":"10.1002","volume":"45","author":[{"given":"Kamlesh","family":"Shrivas","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hui\u2010Fen","family":"Wu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2010,11,4]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac00171a028"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.1192"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/humu.20023"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac051162m"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac00119a021"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac702309w"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.3192"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.1290020802"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.3364"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.3462"},{"key":"e_1_2_7_12_2","doi-asserted-by":"publisher","DOI":"10.1002\/jms.1450"},{"key":"e_1_2_7_13_2","doi-asserted-by":"publisher","DOI":"10.1002\/anie.200603917"},{"key":"e_1_2_7_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.200600496"},{"key":"e_1_2_7_15_2","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.3681"},{"key":"e_1_2_7_16_2","doi-asserted-by":"publisher","DOI":"10.1021\/jp075159d"},{"key":"e_1_2_7_17_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja043907w"},{"key":"e_1_2_7_18_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac802042x"},{"key":"e_1_2_7_19_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac0711440"},{"key":"e_1_2_7_20_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-006-0427-0"},{"key":"e_1_2_7_21_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac702618h"},{"key":"e_1_2_7_22_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac050213c"},{"key":"e_1_2_7_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jasms.2004.11.017"},{"key":"e_1_2_7_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jasms.2004.08.001"},{"key":"e_1_2_7_25_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac061154l"},{"key":"e_1_2_7_26_2","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.3710"},{"key":"e_1_2_7_27_2","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.3363"},{"key":"e_1_2_7_28_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac8015664"},{"key":"e_1_2_7_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2008.09.011"},{"key":"e_1_2_7_30_2","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200800772"},{"key":"e_1_2_7_31_2","doi-asserted-by":"publisher","DOI":"10.1021\/jp991937t"},{"key":"e_1_2_7_32_2","doi-asserted-by":"publisher","DOI":"10.1038\/382607a0"},{"key":"e_1_2_7_33_2","doi-asserted-by":"publisher","DOI":"10.1038\/27873"},{"key":"e_1_2_7_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcrysgro.2004.12.031"},{"key":"e_1_2_7_35_2","doi-asserted-by":"publisher","DOI":"10.1002\/adma.200305661"},{"key":"e_1_2_7_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mseb.2007.03.010"},{"key":"e_1_2_7_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-0248(03)01198-9"},{"key":"e_1_2_7_38_2","doi-asserted-by":"publisher","DOI":"10.1002\/adma.200500418"},{"key":"e_1_2_7_39_2","doi-asserted-by":"publisher","DOI":"10.1088\/0957-4484\/15\/12\/029"},{"key":"e_1_2_7_40_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0958-6946(00)00065-0"},{"key":"e_1_2_7_41_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.matchemphys.2004.11.041"},{"key":"e_1_2_7_42_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0008-6223(03)00339-7"},{"key":"e_1_2_7_43_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.matlet.2005.01.042"}],"container-title":["Journal 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