{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,17]],"date-time":"2025-10-17T00:34:52Z","timestamp":1760661292711,"version":"build-2065373602"},"reference-count":40,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2011,10,5]],"date-time":"2011-10-05T00:00:00Z","timestamp":1317772800000},"content-version":"vor","delay-in-days":4,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Mass. Spectrom."],"published-print":{"date-parts":[[2011,10]]},"abstract":"<jats:p>Disaturated\u2010phosphatidylcholine (DSPC) and phosphatidylglycerol (PG) are respectively the first and the third most abundant phospholipid in human alveolar surfactant. Their concentration decreases in airway surfactant of adults and infants with respiratory distress syndrome and cystic fibrosis. In this study, we used mass spectrometry (IRMS) to investigate the turnover of DSPC and PG in tracheal aspirates (TA) obtained from infants with normal or diseased lungs. We studied eight infants requiring mechanical ventilation: two with no lung disease, four with diaphragmatic hernia, one with ATP\u2010binding cassette sub\u2010family A member 3 heterozygote mutation and one with sepsis. Patients received deuterated water for 48\u2009h as metabolic precursors of palmitate\u2010DSPC and palmitate\u2010PG. Serial TAs were obtained every 6\u2009h for five days or until extubation. DSPC and PG were isolated from TA by column and high\u2010performance thin layer chromatography. Deuterium enrichments of palmitate\u2010DSPC and PG residues were measured by IRMS coupled with a gas chromatographer. Median secretion time (ST), peak time (PT) and fractional synthesis rate (FSR) were 3.7 [0.9\u2013 13.4] h, 71.0 [52.2 \u2013 85.2] h and 6.6 [6.3 \u2013 11.1] %\/day for DSPC and 19.3 [6.4 \u2013 22.8] h, 49.0 [33.0 \u2013 52.5] h and 5.8 [4.8 \u2013 10.9] %\/day for PG. This study shows that it is feasible to use deuterium derived from body water to trace simultaneously airway surfactant DSPC and PG in humans. When compared within the same patient, DSPC and PG had similar fractional synthesis rates, but PG had a shorter PT, suggesting differences in the life cycle of these essential surfactant components. Copyright \u00a9 2011 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/jms.1979","type":"journal-article","created":{"date-parts":[[2011,10,6]],"date-time":"2011-10-06T00:07:13Z","timestamp":1317859633000},"page":"986-992","source":"Crossref","is-referenced-by-count":10,"title":["Simultaneous measurement of phosphatidylglycerol and disaturated\u2010phosphatidylcholine palmitate kinetics from alveolar surfactant. Study in infants with stable isotope tracer, coupled with isotope ratio mass spectrometry"],"prefix":"10.1002","volume":"46","author":[{"given":"Luca","family":"Vedovelli","sequence":"first","affiliation":[{"name":"Department of Anesthesiology and Pharmacology University of Padua  Padua Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aldo","family":"Baritussio","sequence":"additional","affiliation":[{"name":"Department of Medical and Surgical Sciences University of Padua  Padua Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Virgilio Paolo","family":"Carnielli","sequence":"additional","affiliation":[{"name":"Department of Maternal and Infantile Sciences Polytechnic University of Marche  Ancona Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manuela","family":"Simonato","sequence":"additional","affiliation":[{"name":"Department of Pediatrics University of Padua  Padua Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pietro","family":"Giusti","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology and Pharmacology University of Padua  Padua Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paola Elisa","family":"Cogo","sequence":"additional","affiliation":[{"name":"Cardiac Intensive Care, Department of Pediatric Cardiology and Cardiosurgery \u2018Bambino Ges\u00f9\u2019 Pediatric Hospital  Rome Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2011,10,5]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0925-4439(98)00061-1"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4757-5806-1_11"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1139\/o84-146"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm.149.3.7509706"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm.163.1.9903029"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1186\/1465-9921-8-55"},{"key":"e_1_2_6_8_1","doi-asserted-by":"crossref","first-page":"1156","DOI":"10.1183\/09031936.93.06081156","article-title":"Distearoyl phosphatidylglycerol liposomes improve surface and transport properties of CF mucus","volume":"6","author":"Girod de Bentzmann S.","year":"1993","journal-title":"Eur. 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