{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T00:28:12Z","timestamp":1761611292162,"version":"build-2065373602"},"reference-count":26,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2011,11,11]],"date-time":"2011-11-11T00:00:00Z","timestamp":1320969600000},"content-version":"vor","delay-in-days":10,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Mass. Spectrom."],"published-print":{"date-parts":[[2011,11]]},"abstract":"<jats:p>A rapid, selective and sensitive method for quantification of latanoprost free acid in rabbit aqueous humor (AH) and ciliary body (CB) using reverse phase\u2010high performance liquid chromatography coupled with electrospray ionization (ESI)\u2010mass spectrometry\/mass spectrometry has been developed and validated. Quantification in AH and CB was achieved by stable isotope dilution employing tetra\u2010deuterated analog of latanoprost free acid, used as internal standard. Sample preparation was based on protein precipitation with methanol in AH, and on liquid extraction with a mixture of ethyl acetate and isopropanol 60:40 (v\/v) in CB. Elution was achieved on an octylsilica (C8) column, using an isocratic elution method. Detection was performed on a triple quadrupole mass spectrometer, using ESI in positive ion selected reaction monitoring mode. Calibration curves were linear in the validated concentration ranges of 10\u2013160\u2009ng\/mL in AH and 80\u20131280\u2009ng\/g in CB. The accuracy and precision values, obtained from three different sets of quality control samples, each analyzed in triplicate on three different days, were within the generally accepted criteria for analytical methods (&lt; 15%). The limit of detection was 30.66\u2009pg\/mL in AH and 237.75\u2009pg\/g in CB. The assay proved to be accurate and precise when applied to the <jats:italic>in vivo<\/jats:italic> study of latanoprost free acid in rabbit AH and CB after single administration of an eye drops containing latanoprost. Copyright \u00a9 2011 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/jms.2004","type":"journal-article","created":{"date-parts":[[2011,11,16]],"date-time":"2011-11-16T18:49:40Z","timestamp":1321469380000},"page":"1168-1174","source":"Crossref","is-referenced-by-count":6,"title":["Development and validation of a liquid chromatography\/electrospray ionization tandem mass spectrometry method for the quantification of latanoprost free acid in rabbit aqueous humor and ciliary body"],"prefix":"10.1002","volume":"46","author":[{"given":"Alessio","family":"Zammataro","sequence":"first","affiliation":[{"name":"SIFI SpA Via Ercole Patti 36  95020 Lavinaio \u2010 Aci S. 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