{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T00:05:06Z","timestamp":1791504306688,"version":"4.3.4"},"reference-count":50,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2012,3,8]],"date-time":"2012-03-08T00:00:00Z","timestamp":1331164800000},"content-version":"vor","delay-in-days":7,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Mass. Spectrom."],"published-print":{"date-parts":[[2012,3]]},"abstract":"<jats:p>This study focuses on acquiring information on the degradation process of proteinaceous binders due to ultra violet (UV) radiation and possible interactions owing to the presence of historical mineral pigments. With this aim, three different paint model samples were prepared according to medieval recipes, using rabbit glue as proteinaceus binders. One of these model samples contained only the binder, and the other two were prepared by mixing each of the pigments (cinnabar or azurite) with the binder (glue tempera model samples). The model samples were studied by applying Principal Component Analysis (PCA) to their mass spectra obtained with Matrix\u2010Assisted Laser Desorption\/Ionization\u2010Time of Flight Mass Spectrometry (MALDI\u2010TOF\u2010MS). The complementary use of Fourier Transform Infrared Spectroscopy to study conformational changes of secondary structure of the proteinaceous binder is also proposed. Ageing effects on the model samples after up to 3000\u2009h of UV irradiation were periodically analyzed by the proposed approach. PCA on MS data proved capable of identifying significant changes in the model samples, and the results suggested different aging behavior based on the pigment present. This research represents the first attempt to use this approach (PCA on MALDI\u2010TOF\u2010MS data) in the field of Cultural Heritage and demonstrates the potential benefits in the study of proteinaceous artistic materials for purposes of conservation and restoration. Copyright \u00a9 2012 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/jms.2966","type":"journal-article","created":{"date-parts":[[2012,3,14]],"date-time":"2012-03-14T23:53:04Z","timestamp":1331769184000},"page":"322-330","source":"Crossref","is-referenced-by-count":29,"title":["Collagen\u2010based proteinaceous binder\u2010pigment interaction study under UV ageing conditions by MALDI\u2010TOF\u2010MS and principal component analysis"],"prefix":"10.1002","volume":"47","author":[{"given":"Julia","family":"Romero\u2010Pastor","sequence":"first","affiliation":[{"name":"Department of Mineralogy and Petrology University of Granada  Campus Fuentenueva s\/n 18071 Granada Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natalia","family":"Navas","sequence":"additional","affiliation":[{"name":"Department of Analytical Chemistry University of Granada  Campus Fuentenueva s\/n 18071 Granada Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stepanka","family":"Kuckova","sequence":"additional","affiliation":[{"name":"Charles University Department of Chemistry and Chemical Education  M.D. Rettigov\u00e9 4 116 39 Prague 1 Czech Republic"},{"name":"Institute of Chemical Technology Department of Biochemistry and Biotechnology  Technick\u00e1 5 166 28 Praha 6 Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alejandro","family":"Rodr\u00edguez\u2010Navarro","sequence":"additional","affiliation":[{"name":"Department of Mineralogy and Petrology University of Granada  Campus Fuentenueva s\/n 18071 Granada Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carolina","family":"Cardell","sequence":"additional","affiliation":[{"name":"Department of Mineralogy and Petrology University of Granada  Campus Fuentenueva s\/n 18071 Granada Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,3,8]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0003-2670(99)00827-2"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac8019152"},{"key":"e_1_2_6_4_1","volume-title":"El arte de la pintura","author":"Pacheco F.","year":"1990"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac1027275"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0026-265X(00)00075-8"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(01)00914-1"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2006.04.005"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac070373j"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/jrs.1951"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2008.10.014"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vibspec.2010.04.003"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.2307\/3179992"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.2307\/1506634"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/jms.145"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-009-2653-8"},{"key":"e_1_2_6_17_1","volume-title":"Molecular Cell Biology","author":"Lodish H.","year":"2000"},{"key":"e_1_2_6_18_1","doi-asserted-by":"crossref","first-page":"95","DOI":"10.3109\/10409239509085140","article-title":"The use and misuse of FTIR Spectroscopy in the determination of protein structure","volume":"20","author":"Jackson M.","year":"1995","journal-title":"Crc. 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