{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T01:50:10Z","timestamp":1789696210916,"version":"4.0.1"},"reference-count":44,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2012,10,29]],"date-time":"2012-10-29T00:00:00Z","timestamp":1351468800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Mass. Spectrom."],"published-print":{"date-parts":[[2012,11]]},"abstract":"<jats:p>Surfactant protein B (SP\u2010B) plays a key role in surfactant homeostasis affecting its biophysical properties and physiological function.<\/jats:p><jats:p>Recently, a method to measure SP\u2010B amount and kinetics from tracheal aspirates (TAs) became available. The main objective of this study was to improve the critical steps of the procedure to obtain a better SP\u2010B sensitivity.<\/jats:p><jats:p>We administered a 24\u2009h continuous infusion of 1\u2009mg\/kg\/h of 1<jats:sup>13<\/jats:sup>\u2009C\u2010leucine to ten newborn infants. SP\u2010B was isolated from serial TAs and its fractional synthesis rate, secretion time, peak time and half life were derived from <jats:sup>13<\/jats:sup>\u2009C enrichment curves obtained by gas chromatography mass spectrometry. SP\u2010B amount in TAs was also assessed.<\/jats:p><jats:p>During the extraction step, acidification and organic solvent ratio optimization doubled the recovery of SP\u2010B from TAs, so did the elongation of the propylation time (from 20\u2009min to 1\u2009h) with enhanced leucine derivatization yield.<\/jats:p><jats:p>Measurement of <jats:sup>13<\/jats:sup>\u2009C leucine enrichments, and therefore all SP\u2010B kinetics parameters, were successfully calculated in all TAs samples due to the increase of SP\u2010B yield.<\/jats:p><jats:p>SP\u2010B amount was 0.29 (0.16\u20130.41) % of total phospholipids with a minimum value of 0.08% belonging to one of the respiratory distress syndrome (RDS) patients.<\/jats:p><jats:p>In conclusion, this new procedure enables accurate determination of SP\u2010B kinetics even in the presence of low protein amount like in preterm RDS patients. Copyright \u00a9 2012 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/jms.3065","type":"journal-article","created":{"date-parts":[[2012,11,7]],"date-time":"2012-11-07T20:25:06Z","timestamp":1352319906000},"page":"1415-1419","source":"Crossref","is-referenced-by-count":7,"title":["Surfactant protein B amount and kinetics in newborn infants: an optimized procedure"],"prefix":"10.1002","volume":"47","author":[{"given":"Manuela","family":"Simonato","sequence":"first","affiliation":[{"name":"Department of Medicine, Anaesthesia and Critical Care University of Padova  Padova Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aldo","family":"Baritussio","sequence":"additional","affiliation":[{"name":"Department of Medical and Surgical Sciences University of Padova  Padova Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luca","family":"Vedovelli","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Anaesthesia University of Padova  Padova Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giulia","family":"Lamonica","sequence":"additional","affiliation":[{"name":"Women's and Children's Health Department University of Padova  Padova Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Virgilio Paolo","family":"Carnielli","sequence":"additional","affiliation":[{"name":"Neonatal Division &amp; Pediatric Surgery Division, Institute of Maternal\u2010Infantile Sciences Polytechnic University of Marche and University of Ancona  Ancona Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paola Elisa","family":"Cogo","sequence":"additional","affiliation":[{"name":"Department of Medical Cardiac &amp; Cardiac Surgical Pediatrics Bambino Ges\u00f9 Children's Hospital  Roma Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,10,29]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.physiol.63.1.555"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra022387"},{"issue":"4","key":"e_1_2_5_4_1","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1159\/000078169","article-title":"Surfactant protein B: structure and function","volume":"85","author":"Hawgood S.","year":"2004","journal-title":"Biol. 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