{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T20:36:09Z","timestamp":1761510969790,"version":"build-2065373602"},"reference-count":35,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2013,1,9]],"date-time":"2013-01-09T00:00:00Z","timestamp":1357689600000},"content-version":"vor","delay-in-days":8,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Mass. Spectrom."],"published-print":{"date-parts":[[2013,1]]},"abstract":"<jats:p><jats:italic>Chelidonium majus<\/jats:italic> L. is one of the most important medicinal plants of the family <jats:italic>Papaveraceae<\/jats:italic>. Its pharmacological effects have been primarily attributed to the presence of a number of alkaloids. In the present study, a sensitive and selective liquid chromatography\u2212tandem mass spectrometry method for simultaneous determination of five isoquinoline alkaloids from <jats:italic>Chelidonium majus<\/jats:italic> L. was developed and validated. The analytes (protopine, chelidonine, coptisine, sanguinarine and chelerythrine), together with the internal standard (palmatine), were extracted from acidified rat plasma with ethyl acetate\u2212dichloromethane (4:1, <jats:italic>v<\/jats:italic>\/<jats:italic>v<\/jats:italic>). Chromatographic separation was carried out on a Diamonsil C<jats:sub>18<\/jats:sub> column with an isocratic mobile phase consisting of acetonitrile and water (adjusted to pH 2.3 with formic acid) (30:70, <jats:italic>v<\/jats:italic>\/<jats:italic>v<\/jats:italic>) at a flow rate of 0.4\u2009ml\/min. Mass spectrometric detection was performed by selected reaction monitoring mode via electrospray ionization source operating in positive ionization mode. The assay exhibited good linearity (<jats:italic>r<\/jats:italic>\u2009\u2265\u20090.9933) for all the analytes. The lower limits of quantification were 0.197\u22121.27\u2009ng\/ml using only 50 \u00b5l of plasma sample. The intra\u2010 and inter\u2010day precisions were less than 11.9%, and the accuracy was between \u22126.3% and 9.3%. The method was successfully applied to the pharmacokinetic study of the five alkaloids in rats after intragastric administration of <jats:italic>Chelidonium majus<\/jats:italic> L. extract. 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