{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T18:27:24Z","timestamp":1760984844971,"version":"build-2065373602"},"reference-count":29,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2013,9,20]],"date-time":"2013-09-20T00:00:00Z","timestamp":1379635200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Mass Spectrom."],"published-print":{"date-parts":[[2013,10]]},"abstract":"<jats:p>We report herein the glycation sites in a vaccine candidate for cholera formed by conjugation of the synthetic hexasaccharide fragment of the O\u2010specific polysaccharide of <jats:italic>Vibrio cholerae<\/jats:italic>, serotype Ogawa, to the recombinant tetanus toxin C\u2010fragment (rTT\u2013Hc) carrier. Matrix\u2010assisted laser desorption\/ionization time\u2010of\u2010flight mass spectrometry analysis of the vaccine revealed that it is composed of a mixture of neoglycoconjugates with carbohydrate\u2009:\u2009protein ratios of 1.9\u2009:\u20091, 3.0\u2009:\u20091, 4.0\u2009:\u20091, 4.9\u2009:\u20091, 5.9\u2009:\u20091, 6.9\u2009:\u20091, 7.9\u2009:\u20091 and 9.1\u2009:\u20091. Liquid chromatography tandem mass spectrometry (LC\u2010MS\/MS) analysis of the tryptic and GluC V8 digests allowed identification of 12 glycation sites in the carbohydrate\u2013protein neoglycoconjugate vaccine. The glycation sites are located exclusively on lysine (Lys) residues and are listed as follows: Lys 22, Lys 61, Lys 145, Lys 239, Lys 278, Lys 318, Lys 331, Lys 353, Lys 378, Lys 389, Lys 396 and Lys 437. Based on the 3\u2010D representation of the rTT\u2013Hc protein, all the glycation sites correspond to lysines located at the outer surface of the protein. Copyright \u00a9 2013 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/jms.3258","type":"journal-article","created":{"date-parts":[[2013,10,3]],"date-time":"2013-10-03T20:17:42Z","timestamp":1380831462000},"page":"1083-1090","source":"Crossref","is-referenced-by-count":13,"title":["Mapping the glycation sites in the neoglycoconjugate from hexasaccharide antigen of <i>Vibrio cholerae<\/i>, serotype <i>Ogawa<\/i> and the recombinant tetanus toxin C\u2010fragment carrier"],"prefix":"10.1002","volume":"48","author":[{"given":"Farid","family":"Jahouh","sequence":"first","affiliation":[{"name":"Memorial University Biochemistry Department  St John's NL Canada A1B 3X7"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peng","family":"Xu","sequence":"additional","affiliation":[{"name":"NIH, NIDDK, LBC  Bethesda MD 20892\u20100815 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Willie F.","family":"Vann","sequence":"additional","affiliation":[{"name":"Laboratory of Bacterial Toxins OVRR, CBER, FDA  8800 Rockville Pike Bethesda MD 20892 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pavol","family":"Kov\u00e1\u010d","sequence":"additional","affiliation":[{"name":"NIH, NIDDK, LBC  Bethesda MD 20892\u20100815 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joseph H.","family":"Banoub","sequence":"additional","affiliation":[{"name":"Memorial University Biochemistry Department  St John's NL Canada A1B 3X7"},{"name":"Fisheries and Oceans Canada Science Branch, Special Projects  St John's NL Canada A1C 5X1"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,9,20]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.2174\/1385272003376111"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/1099-0690(200111)2001:22<4331::AID-EJOC4331>3.0.CO;2-R"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1517\/17460441.2011.609554"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.carres.2007.10.015"},{"key":"e_1_2_5_6_1","first-page":"69","volume-title":"Vaccine Design: Innovative Approaches and Novel Strategies","author":"Bundle D. 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