{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T08:32:02Z","timestamp":1777624322390,"version":"3.51.4"},"reference-count":18,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2014,12,5]],"date-time":"2014-12-05T00:00:00Z","timestamp":1417737600000},"content-version":"vor","delay-in-days":4,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Mass Spectrom."],"published-print":{"date-parts":[[2014,12]]},"abstract":"<jats:p>Drugs deposited in human teeth are well preserved; the spectrum of toxicological investigations may therefore be supplemented by an analysis method for drugs in teeth. A liquid chromatography\u2013electrospray ionization tandem mass spectrometry assay for the detection and quantification of basic drugs of abuse in bovine dentin samples was developed and validated. The drugs and metabolites amphetamine, methamphetamine, methylenedioxymethylamphetamine, methylenedioxyethylamphetamine, codeine, morphine, cocaine and benzoylecgonine were extracted from 50 mg ground dentin powder by ultrasonication for 60 min in methanol 3 times. The extracts were analyzed on a triple\u2010quadrupole mass\u2010spectrometer in multiple reaction monitoring mode. The method was validated and proved to be accurate, precise, selective, specific and stable with good linearity within the calibration range and a lower limit of quantification of 10 to 20\u2009pg\/mg. To artificially load bovine dentin samples with drugs, the natural process of de\u2010 and remineralization in the oral cavity was mimicked by a pH\u2010cycling experiment. The artificially drug\u2010loaded dentin samples showed drug concentrations of 20 to 80\u2009pg\/mg. The method can be applied in further <jats:italic>in vitro<\/jats:italic> experiments as well as in post\u2010mortem cases, especially where limited sample tissue is available. Copyright \u00a9 2014 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/jms.3464","type":"journal-article","created":{"date-parts":[[2014,12,5]],"date-time":"2014-12-05T22:52:24Z","timestamp":1417819944000},"page":"1306-1313","source":"Crossref","is-referenced-by-count":16,"title":["Determination of drugs of abuse in bovine dentin using liquid chromatography\u2013electrospray ionization tandem mass spectrometry"],"prefix":"10.1002","volume":"49","author":[{"given":"J.","family":"Spinner","sequence":"first","affiliation":[{"name":"Institute of Forensic Medicine University Medical Center Freiburg  Freiburg Germany"},{"name":"Institute of Forensic Medicine University of Bern  Bern Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Klima","sequence":"additional","affiliation":[{"name":"Institute of Forensic Medicine University Medical Center Freiburg  Freiburg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Kempf","sequence":"additional","affiliation":[{"name":"Institute of Forensic Medicine University Medical Center Freiburg  Freiburg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L. 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