{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,21]],"date-time":"2026-01-21T09:26:43Z","timestamp":1768987603749,"version":"3.49.0"},"reference-count":28,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2014,9,10]],"date-time":"2014-09-10T00:00:00Z","timestamp":1410307200000},"content-version":"vor","delay-in-days":9,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Mass Spectrom."],"published-print":{"date-parts":[[2014,9]]},"abstract":"<jats:p>\u03b2<jats:sub>2<\/jats:sub>\u2010agonists are often abused in cattle breeding because of their effects on animal growth and meat properties. The use of \u03b2<jats:sub>2<\/jats:sub>\u2010agonists as growth promoters is forbidden in the European Union (Council Directive 96\/23\/EC classifies them into group A of Annex I), due to their toxicity and carcinogenic properties, as for anabolic steroids, which are often administered in combination with \u03b2<jats:sub>2<\/jats:sub>\u2010agonists, to promote the storage of proteins and increase muscle size.<\/jats:p><jats:p>A unique confirmatory liquid chromatography\u2013tandem mass spectrometry (LC\u2010MS\/MS) method for the quantitative detection of 13 \u03b2<jats:sub>2<\/jats:sub>\u2010agonists and anabolic steroids plus the qualitative identification of other three analytes in bovine hair was developed and validated, according to Decision 2002\/657\/CE. Hair samples were washed with dichloromethane, digested within a NaOH solution and subjected to liquid\u2013liquid extraction. The analysis was performed by high performance liquid chromatography coupled to a triple quadrupole mass spectrometer operating in the selected reaction monitoring mode. The absence of matrix interferents, together with good repeatability of both retention times and relative abundances of diagnostic transitions, allowed the correct identification of all analytes. The quantitative calibrations obtained from spiked blank hair samples proved linear in the range tested. CC\u03b1 and CC\u03b2 ranged from 0.5\u2009ng\/g to 30\u2009ng\/g. Intralaboratory reproducibility (CV%) ranged between 5.0 and 17.7 and trueness between 96%\u2009\u00b1\u20097% and 105%\u2009\u00b1\u20098%. The applicability of the method to real positive samples was demonstrated for both \u03b2<jats:sub>2<\/jats:sub>\u2010agonists and anabolic steroids.<\/jats:p><jats:p>17\u03b1\u2010boldenone was found in most (70%) hair samples obtained from untreated animals, supporting the hypothesis of endogenous production of this steroid. Copyright \u00a9 2014 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/jms.3467","type":"journal-article","created":{"date-parts":[[2014,9,17]],"date-time":"2014-09-17T06:12:58Z","timestamp":1410934378000},"page":"936-946","source":"Crossref","is-referenced-by-count":15,"title":["Development, validation and application to real samples of a multiresidue LC\u2010MS\/MS method for determination of \u03b2<sub>2<\/sub>\u2010agonists and anabolic steroids in bovine hair"],"prefix":"10.1002","volume":"49","author":[{"given":"M.","family":"Leporati","sequence":"first","affiliation":[{"name":"Centro Regionale Antidoping e di Tossicologia \u2018Alessandro Bertinaria\u2019  regione Gonzole 10\/1 10043 Orbassano Torino Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Bergoglio","sequence":"additional","affiliation":[{"name":"Dipartimento di Chimica Universit\u00e0 degli Studi di Torino  via Pietro Giuria 7 10125 Torino Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.","family":"Capra","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d'Aosta  via Bologna 148 10154 Torino Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E.","family":"Bozzetta","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d'Aosta  via Bologna 148 10154 Torino Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. 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