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Mass Spectrom."],"published-print":{"date-parts":[[2015,3]]},"abstract":"<jats:p>Glycation is a non\u2010enzymatic reaction of protein amino and guanidino groups with reducing sugars or dicarbonyl products of their oxidative degradation. Modification of arginine residues by dicarbonyls such as glyoxal and methylglyoxal results in formation of advanced glycation end\u2010products (AGEs). In mammals, these modifications impact in diabetes mellitus, uremia, atherosclerosis and ageing. However, due to the low abundance of individual AGE\u2010peptides in enzymatic digests, these species cannot be efficiently detected by LC\u2010ESI\u2010MS\u2010based data\u2010dependent acquisition (DDA) experiments.<\/jats:p><jats:p>Here we report an analytical workflow that overcomes this limitation. We describe fragmentation patterns of synthetic AGE\u2010peptides and assignment of modification\u2010specific signals required for unambiguous structure retrieval. Most intense signals were those corresponding to unique fragment ions with <jats:italic>m\/z<\/jats:italic> 152.1 and 166.1, observed in the tandem mass spectra of peptides, containing glyoxal\u2010 and methylglyoxal\u2010derived hydroimidazolone AGEs, respectively. To detect such peptides, specific and sensitive precursor ion scanning methods were established for these signals. Further, these precursor ion scans were incorporated in conventional bottom\u2010up proteomic approach based on data\u2010dependent acquisition (DDA) LC\u2010MS\/MS experiments. The method was successfully applied for the analysis of human serum albumin (HSA) and human plasma protein tryptic digest with subsequent structure confirmation by targeted LC\u2010MS\/MS (DDA). Altogether 44 hydroimidazolone\u2010 and dihydroxyimidazolidine\u2010derived peptides representing 42 AGE\u2010modified proteins were identified in plasma digests obtained from type 2 diabetes mellitus (T2DM) patients. Copyright \u00a9 2015 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/jms.3569","type":"journal-article","created":{"date-parts":[[2015,2,20]],"date-time":"2015-02-20T07:46:53Z","timestamp":1424418413000},"page":"613-624","source":"Crossref","is-referenced-by-count":37,"title":["Specific tandem mass spectrometric detection of AGE\u2010modified arginine residues in peptides"],"prefix":"10.1002","volume":"50","author":[{"given":"Rico","family":"Schmidt","sequence":"first","affiliation":[{"name":"Institute of Bioanalytical Chemistry, Faculty of Chemistry and Mineralogy, Center for Biotechnology and Biomedicine (BBZ) Universit\u00e4t Leipzig  Leipzig Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David","family":"B\u00f6hme","sequence":"additional","affiliation":[{"name":"Institute of Bioanalytical Chemistry, Faculty of Chemistry and Mineralogy, Center for Biotechnology and Biomedicine (BBZ) Universit\u00e4t Leipzig  Leipzig Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David","family":"Singer","sequence":"additional","affiliation":[{"name":"Institute of Bioanalytical Chemistry, Faculty of Chemistry and Mineralogy, Center for Biotechnology and Biomedicine (BBZ) Universit\u00e4t Leipzig  Leipzig Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrej","family":"Frolov","sequence":"additional","affiliation":[{"name":"Institute of Bioanalytical Chemistry, Faculty of Chemistry and Mineralogy, Center for Biotechnology and Biomedicine (BBZ) Universit\u00e4t Leipzig  Leipzig Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,2,18]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-3233(08)60066-2"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1210\/rp.56.1.1"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/ndt\/11.supp5.41"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0096-5332(08)60392-6"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/cber.19590921010"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj3640001"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s002170050137"},{"key":"e_1_2_7_9_1","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1042\/bj3470023","article-title":"Identification of N\n                  \u03c9\u2010carboxymethylarginine as a novel acid\u2010labile advanced glycation end product in collagen","volume":"27","author":"Iijima K.","year":"2000","journal-title":"Biochem. 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