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Mass Spectrom."],"published-print":{"date-parts":[[2015,4]]},"abstract":"<jats:p>Tri\u2010ortho\u2010cresyl phosphate (ToCP) is an anti\u2010wear, flame retardant additive used in industrial lubricants, hydraulic fluids and gasoline. The neurotoxic effects of ToCP arise from the liver\u2010activated metabolite 2\u2010(o\u2010cresyl)\u20104H\u20101,3,2\u2010benzodioxaphosphoran\u20102\u2010one (cresyl saligenin phosphate or CBDP), which inhibits esterase enzymes including butyrylcholinesterase (BChE). Following BChE adduction, CBDP undergoes hydrolysis to form the aged adduct ortho\u2010cresyl phosphoserine (oCP\u2010BChE), thus providing a biomarker of CBDP exposure. Previous studies have identified ToCP in aircraft cabin and cockpit air, but assessing human exposure has been hampered by the lack of a laboratory assay to confirm exposure. This work presents the development of an immunomagnetic\u2010UHPLC\u2010MS\/MS method for the quantitation of unadducted BChE and the long\u2010term CBDP biomarker, oCP\u2010BChE, in human serum. The method has a reportable range from 2.0\u2009ng\/ml to 150\u2009ng\/ml, which is consistent with the sensitivity of methods used to detect organophosphorus nerve agent protein adducts. The assay demonstrated high intraday and interday accuracy (\u226585%) and precision (RSD\u2009\u2264\u200915%) across the calibration range. The method was developed for future analyses of potential human exposure to CBDP. Analysis of human serum inhibited <jats:italic>in vitro<\/jats:italic> with CBDP demonstrated that the oCP\u2010BChE adduct was stable for at least 72\u2009h at 4, 22 and 37\u2009\u00b0C. Compared to a previously reported assay, this method requires 75% less sample volume, reduces analysis time by a factor of 20 and demonstrates a threefold improvement in sensitivity. Published 2015. 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