{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T14:59:44Z","timestamp":1790693984343,"version":"4.1.0"},"reference-count":27,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2016,9,1]],"date-time":"2016-09-01T00:00:00Z","timestamp":1472688000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Italian Health Ministry"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Mass Spectrom."],"published-print":{"date-parts":[[2016,9]]},"abstract":"<jats:p>\n                    A multiclass method for screening and confirmatory analysis of antimicrobial residues in milk has been developed and validated. Sixty\u2010two antibiotics belonging to ten different drug families (amphenicols, cephalosporins, lincosamides, macrolides, penicillin, pleuromutilins, quinolones, rifamycins, sulfonamides and tetracyclines) have been included. After the addition of an aqueous solution of EDTA, the milk samples were extracted twice with acetonitrile, evaporated and dissolved in ammonium acetate. After centrifugation, 10\u2009\u00b5l were analysed using LC\u2010Q\u2010Orbitrap operating in positive electrospray ionization mode. The method was validated in bovine milk in the range 2\u2013150\u2009\u00b5g\u2009kg\n                    <jats:sup>\u22121<\/jats:sup>\n                    for all antibiotics; for four compounds with maximum residue limits higher than 100\u2009\u00b5g\u2009kg\n                    <jats:sup>\u22121<\/jats:sup>\n                    , the validation interval has been extended until 333\u2009\u00b5g\u2009kg\n                    <jats:sup>\u22121<\/jats:sup>\n                    . The estimated performance characteristics were satisfactory complying with the requirements of Commission Decision 2002\/657\/EC. Good accuracies were obtained also taking advantage from the versatility of the hybrid mass analyser. Identification criteria were achieved verifying the mass accuracy and ion ratio of two ions, including the pseudomolecular one, where possible. Finally, the developed procedure was applied to 13 real cases of suspect milk samples (microbiological assay) confirming the presence of one or more antibiotics, although frequently, the maximum residue limits were not exceeded. The availability of rapid multiclass confirmatory methods can avoid wastes of suspect, but compliant, raw milk samples. Copyright \u00a9 2016 John Wiley &amp; Sons, Ltd.\n                  <\/jats:p>","DOI":"10.1002\/jms.3834","type":"journal-article","created":{"date-parts":[[2016,8,4]],"date-time":"2016-08-04T10:49:02Z","timestamp":1470307742000},"page":"792-804","source":"Crossref","is-referenced-by-count":47,"title":["Multiclass method for the determination of 62 antibiotics in milk"],"prefix":"10.1002","volume":"51","author":[{"given":"Simone","family":"Moretti","sequence":"first","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale dell'Umbria e delle Marche  Via G. Salvemini, 1 06126 Perugia Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriele","family":"Cruciani","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Biology and Biotechnology University of Perugia  Via Elce di Sotto 10 06123 Perugia Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sara","family":"Romanelli","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale dell'Umbria e delle Marche  Via G. Salvemini, 1 06126 Perugia Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosanna","family":"Rossi","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale dell'Umbria e delle Marche  Via G. Salvemini, 1 06126 Perugia Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giorgio","family":"Saluti","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale dell'Umbria e delle Marche  Via G. 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