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Mass Spectrom."],"published-print":{"date-parts":[[2017,2]]},"abstract":"<jats:p>The ability of a thiol\u2010containing molecule, thiosalicylic acid (TSA), to function as a reactive matrix for matrix\u2010assisted laser desorption\/ionization (MALDI) mass spectrometry analysis of peptides has been investigated. Although TSA has reducing characteristics, the use of TSA did not cause a reduction\u2010induced MALDI in\u2010source decay, probably because of the weak interactions between the thiol group in TSA and the carboxyl oxygen in the peptide. In contrast, when peptides containing disulfide bonds were analyzed by MALDI with TSA as the matrix, the disulfide bond was partially cleaved owing to the reaction with TSA, producing TSA\u2010adducted peptides. The reaction between the disulfide bond and TSA was suggested to be occurred in solution. The comparison of the MALDI mass spectra obtained using conventional matrix and TSA allows us to count the number of disulfide bonds in the peptides. Copyright \u00a9 2017 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/jms.3906","type":"journal-article","created":{"date-parts":[[2017,1,10]],"date-time":"2017-01-10T23:43:25Z","timestamp":1484091805000},"page":"127-131","source":"Crossref","is-referenced-by-count":5,"title":["Direct MALDI\u2010MS analysis of the disulfide bonds in peptide using thiosalicylic acid as a reactive matrix"],"prefix":"10.1002","volume":"52","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9357-8420","authenticated-orcid":false,"given":"D.","family":"Asakawa","sequence":"first","affiliation":[{"name":"National Institute of Advanced Industrial Science and Technology (AIST)  Tsukuba Central 2, 1\u20101\u20101 Umezono Tsukuba Ibaraki 305\u20138568 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I.","family":"Osaka","sequence":"additional","affiliation":[{"name":"Center for Nano Materials and Technology Japan Advanced Institute of Science and Technology  1\u20131 Asahidai Nomi Ishikawa 923\u20131211 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,1,31]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/mas.21444"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13361-011-0131-y"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13361-012-0523-7"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/jms.3182"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1021\/jp311464k"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13361-014-0868-1"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac403285b"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/jms.3748"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac00117a027"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac070849z"},{"key":"e_1_2_5_12_1","volume-title":"Gaussian 09; Revision D.01","author":"Frisch M. 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