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This is the case of toads, characterized by the presence of a pair of parotoid macroglands, strategically located to give protection by poison release in case of attacks. The product secreted consists of a wide variety of chemical compounds including proteins, peptides, biogenic amines, toxic steroidal bufadienolides, and various alkaloids, depending on the species. In this work, using <jats:styled-content style=\"fixed-case\"><jats:italic>Rhinella arenarum<\/jats:italic>,<\/jats:styled-content> we have performed, for the first time, the matrix assisted\u2010ultraviolet laser desorption\/ionization mass spectrometry and tandem mass spectrometry characterization of the components of the secretion used as crude material, just suspended in MeOH (or MeCN). The crude sample as a whole (whole suspension) was spotted on the matrix assisted\u2010ultraviolet laser desorption plate for analysis. Electrospray ionization\u2010Orbitrap was used for cross\u2010checking experiments. The pattern of signals obtained at m\/z ranges 600 to 800 and 1200 to 1600 could be assigned as the argininyl bufadienolide esters fingerprint characteristic of female and male. Variation patterns for gender (female, male), age (non\u2010reproductive, reproductive), and season (non\u2010reproductive, reproductive) are described.<\/jats:p>","DOI":"10.1002\/jms.4082","type":"journal-article","created":{"date-parts":[[2018,3,10]],"date-time":"2018-03-10T00:22:41Z","timestamp":1520641361000},"page":"465-475","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["MALDI\u2010MS argininyl bufadienolide esters fingerprint from parotoid gland secretions of <scp><i>Rhinella arenarum<\/i><\/scp>: Age, gender, and seasonal variation"],"prefix":"10.1002","volume":"53","author":[{"given":"Gabriela","family":"Petroselli","sequence":"first","affiliation":[{"name":"Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales Departamento de Qu\u00edmica Org\u00e1nica  Pabell\u00f3n II, 3er P., Ciudad Universitaria 1428 Buenos Aires Argentina"},{"name":"CONICET, Universidad de Buenos Aires. Centro de Investigaci\u00f3n en Hidratos de Carbono (CIHIDECAR) Facultad de Ciencias Exactas y Naturales  Pabell\u00f3n II, 3er P. Ciudad Universitaria 1428 Buenos Aires Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marilina","family":"Raices","sequence":"additional","affiliation":[{"name":"CONICET, Universidad de Buenos Aires. Instituto de Biodiversidad y Biolog\u00eda Experimental y Aplicada (IBBEA) Facultad de Ciencias Exactas y Naturales  Pabell\u00f3n II, 4to P., Ciudad Universitaria 1428 Buenos Aires Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucas D.","family":"Jungblut","sequence":"additional","affiliation":[{"name":"CONICET, Universidad de Buenos Aires. Instituto de Biodiversidad y Biolog\u00eda Experimental y Aplicada (IBBEA) Facultad de Ciencias Exactas y Naturales  Pabell\u00f3n II, 4to P., Ciudad Universitaria 1428 Buenos Aires Argentina"},{"name":"Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales Departamento de Biodiversidad y Biolog\u00eda Experimental  Pabell\u00f3n II, 4to P., Ciudad Universitaria 1428 Buenos Aires Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea G.","family":"Pozzi","sequence":"additional","affiliation":[{"name":"CONICET, Universidad de Buenos Aires. Instituto de Biodiversidad y Biolog\u00eda Experimental y Aplicada (IBBEA) Facultad de Ciencias Exactas y Naturales  Pabell\u00f3n II, 4to P., Ciudad Universitaria 1428 Buenos Aires Argentina"},{"name":"Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales Departamento de Biodiversidad y Biolog\u00eda Experimental  Pabell\u00f3n II, 4to P., Ciudad Universitaria 1428 Buenos Aires Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0169-0173","authenticated-orcid":false,"given":"Rosa","family":"Erra\u2010Balsells","sequence":"additional","affiliation":[{"name":"Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales Departamento de Qu\u00edmica Org\u00e1nica  Pabell\u00f3n II, 3er P., Ciudad Universitaria 1428 Buenos Aires Argentina"},{"name":"CONICET, Universidad de Buenos Aires. Centro de Investigaci\u00f3n en Hidratos de Carbono (CIHIDECAR) Facultad de Ciencias Exactas y Naturales  Pabell\u00f3n II, 3er P. 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