{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T20:33:55Z","timestamp":1760646835976,"version":"build-2065373602"},"reference-count":34,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2018,6,26]],"date-time":"2018-06-26T00:00:00Z","timestamp":1529971200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Mass Spectrom"],"published-print":{"date-parts":[[2018,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>CYP3A phenotyping provides a means for personalized drug therapy. We focused our attention on the plasma 6\u03b2\u2010hydroxycortisol (6\u03b2\u2010OHF) to cortisol ratio as an index for CYP3A phenotyping. In the present study, we developed a sensitive and reliable method for the simultaneous determination of 6\u03b2\u2010OHF and cortisol in human plasma using high\u2010performance liquid chromatography\/tandem mass spectrometry together with picolinylester derivatization or nonderivatization methods and 6\u03b2\u2010[9,11,12,12\u2010<jats:sup>2<\/jats:sup>H<jats:sub>4<\/jats:sub>]hydroxycortisol and [1,2,4,19\u2010<jats:sup>13<\/jats:sup>C<jats:sub>4<\/jats:sub>]cortisol as internal standards for in vivo CYP3A phenotyping in humans. The lower limits of quantification were 38.513\u00a0pg\/mL for 6\u03b2\u2010OHF and 38.100\u00a0pg\/mL for cortisol. The relative error and relative standard deviation of the lower limits of quantification were\u00a0&lt;5% for both methods. The intra\u2010day and inter\u2010day assay reproducibilities of the determined 6\u03b2\u2010OHF and cortisol concentrations were consistent with the actual amounts added as relative errors and relative standard deviations for both methods, which were &lt;5.4% and &lt;3.9%, respectively. Both methods were applied for the quantification of plasma 6\u03b2\u2010OHF and cortisol concentrations in healthy subjects taking oral contraceptives. The absolute concentrations and time course of 6\u03b2\u2010OHF and cortisol were found to be consistent when measured using the 2 methods. The ratio as an index for in vivo CYP3A activity decreased after 21\u00a0days of taking oral contraceptives for both methods. To the best of our knowledge, this is the first study reporting the detailed investigation of accuracy and precision in the simultaneous measurement of 6\u03b2\u2010OHF and cortisol in human plasma using liquid chromatography\/tandem mass spectrometry coupled with stable isotope dilution method, which can be applied to CYP3A phenotyping.<\/jats:p>","DOI":"10.1002\/jms.4200","type":"journal-article","created":{"date-parts":[[2018,5,15]],"date-time":"2018-05-15T22:41:46Z","timestamp":1526424106000},"page":"665-674","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Sensitive and simultaneous quantitation of 6\u03b2\u2010hydroxycortisol and cortisol in human plasma by LC\u2010MS\/MS coupled with stable isotope dilution method"],"prefix":"10.1002","volume":"53","author":[{"given":"Ryohei","family":"Hirano","sequence":"first","affiliation":[{"name":"Department of Medicinal Chemistry and Clinical Pharmacy, School of 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