{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T20:52:52Z","timestamp":1790801572533,"version":"4.1.0"},"reference-count":42,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2019,8,16]],"date-time":"2019-08-16T00:00:00Z","timestamp":1565913600000},"content-version":"vor","delay-in-days":15,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"-->NIH\/NIGMS","award":["P41GM103391-07"],"award-info":[{"award-number":["P41GM103391-07"]}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Mass Spectrom"],"published-print":{"date-parts":[[2019,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Matrix\u2010assisted laser desorption\/ionization imaging mass spectrometry (MALDI IMS) is a molecular imaging technology uniquely capable of untargeted measurement of proteins, lipids, and metabolites while retaining spatial information about their location\n                    <jats:italic>in situ<\/jats:italic>\n                    . This powerful combination of capabilities has the potential to bring a wealth of knowledge to the field of molecular histology. Translation of this innovative research tool into clinical laboratories requires the development of reliable sample preparation protocols for the analysis of proteins from formalin\u2010fixed paraffin\u2010embedded (FFPE) tissues, the standard preservation process in clinical pathology. Although ideal for stained tissue analysis by microscopy, the FFPE process cross\u2010links, disrupts, or can remove proteins from the tissue, making analysis of the protein content challenging. To date, reported approaches differ widely in process and efficacy. This tutorial presents a strategy derived from systematic testing and optimization of key parameters, for reproducible\n                    <jats:italic>in situ<\/jats:italic>\n                    tryptic digestion of proteins in FFPE tissue and subsequent MALDI IMS analysis. The approach describes a generalized method for FFPE tissues originating from virtually any source.\n                  <\/jats:p>","DOI":"10.1002\/jms.4384","type":"journal-article","created":{"date-parts":[[2019,6,28]],"date-time":"2019-06-28T22:54:00Z","timestamp":1561762440000},"page":"716-727","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":44,"title":["A recommended and verified procedure for\n                    <i>in situ<\/i>\n                    tryptic digestion of formalin\u2010fixed paraffin\u2010embedded tissues for analysis by matrix\u2010assisted laser desorption\/ionization imaging mass spectrometry"],"prefix":"10.1002","volume":"54","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4633-129X","authenticated-orcid":false,"given":"Audra M.","family":"Judd","sequence":"first","affiliation":[{"name":"Mass Spectrometry Research Center Vanderbilt University  Nashville TN USA"},{"name":"Department of Biochemistry 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