{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,17]],"date-time":"2025-10-17T00:25:29Z","timestamp":1760660729241,"version":"build-2065373602"},"reference-count":24,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2019,11,27]],"date-time":"2019-11-27T00:00:00Z","timestamp":1574812800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Mass Spectrom"],"published-print":{"date-parts":[[2019,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The rapid identification and classification of pathogenic microorganisms, including <jats:italic>Salmonella enterica<\/jats:italic>, is important for the surveillance and prevention of foodborne diseases. Matrix\u2010assisted laser desorption\\ionization time\u2010of\u2010flight mass spectrometry (MALDI\u2010TOFMS) has been shown to be an effective tool for the rapid identification of microorganisms. In a previous report, a mass database consisting of 12 biomarker proteins, S8, L15, L17, L21, L25, S7, superoxide dismutase (SodA), peptidylprolyl cis\u2010trans isomerase C, Gns, YibT, YaiA, and YciF, was introduced for the serotyping of <jats:italic>S. enterica<\/jats:italic> via MALDI\u2010MS (<jats:italic>Applied Microbiology and Biotechnology<\/jats:italic>, 2017, 101, 8557\u20108569). However, the reproducibility of peak detection of biomarkers such as SodA at <jats:italic>m\\z<\/jats:italic> 23 000 was poor. We report here an optimized MALDI\u2010MS method for detecting these biomarkers with high sensitivity and reproducibility. The issue was solved by controlling the bacterial concentration at 1 \u00d7 10 to 1 \u00d7 10<jats:sup>2<\/jats:sup> MFU (3 \u00d7 10<jats:sup>6<\/jats:sup> to 3 \u00d7 10<jats:sup>7<\/jats:sup> CFU\\\u03bcL, as calculated from the MFU), using the colony suspension supernatant obtained by centrifugation, and using matrix additives such as methylenediphosphonic acid and <jats:italic>N<\/jats:italic>\u2010decyl\u2010<jats:italic>\u03b2<\/jats:italic>\u2010D\u2010maltopyranoside. We propose that the method including the above steps is one of the best for detecting biomarkers with high sensitivity and reproducibility.<\/jats:p>","DOI":"10.1002\/jms.4469","type":"journal-article","created":{"date-parts":[[2019,11,7]],"date-time":"2019-11-07T15:57:12Z","timestamp":1573142232000},"page":"966-975","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Improved MALDI\u2010MS method for the highly sensitive and reproducible detection of biomarker peaks for the proteotyping of <scp><i>Salmonella<\/i><\/scp> serotypes"],"prefix":"10.1002","volume":"54","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4443-9459","authenticated-orcid":false,"given":"Yuko","family":"Fukuyama","sequence":"first","affiliation":[{"name":"Koichi Tanaka Mass Spectrometry Research Laboratory Shimadzu Corporation  1 Nishinokyo Kuwabara\u2010cho, Nakagyo\u2010ku Kyoto 604\u20108511 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