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Hence, the characterization of bile acid profiles in the brains of subjects with and without liver failure can provide important clues for the potential treatment of HE. Nanoflow ultra\u2010performance liquid chromatography electrospray ionization ion mobility mass spectrometry (UPLC\u2010ESI\u2010IM\u2010MS) is a highly sensitive method for detection of specific molecules, such as bile acids in brain samples, at biologically relevant concentrations. We used UPLC\u2010ESI\u2010IM\u2010MS to characterize bile acid profiles in brain samples from seven \u201chealthy\u201d control rodents and 22 \u201cdiseased\u201d rodents with liver failure (i.e., induced HE). An isomer of trihydroxycholanoyl\u2010taurine was detected in brain tissue samples from both rats and mice with induced HE; however, this isomer was not detected in the brains of healthy rats and mice. Our findings were confirmed by comparing IM arrival times (AT), exact mass measurements (<jats:italic>m\/z<\/jats:italic>), and mass spectral fragmentation patterns of the experimentally observed suspected species to standards of trihydroxycholanoyl\u2010taurine isomers. Moreover, <jats:italic>In Silico<\/jats:italic> Fractionation was employed to provide an additional analytical dimension to verify bile acid identifications.<\/jats:p>","DOI":"10.1002\/jms.4729","type":"journal-article","created":{"date-parts":[[2021,4,5]],"date-time":"2021-04-05T08:49:30Z","timestamp":1617612570000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Trihydroxycholanoyl\u2010taurine in brains of rodents with hepatic encephalopathy"],"prefix":"10.1002","volume":"56","author":[{"given":"Amy N. 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