{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,16]],"date-time":"2026-01-16T08:54:44Z","timestamp":1768553684646,"version":"3.49.0"},"reference-count":31,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2021,12,8]],"date-time":"2021-12-08T00:00:00Z","timestamp":1638921600000},"content-version":"vor","delay-in-days":7,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Mass Spectrom"],"published-print":{"date-parts":[[2021,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Dried blood spot (DBS) sampling is a minimally invasive method used to collect blood samples of any population for personalized medicine. We aimed to develop a sensitive and reliable analytical method for measuring 6\u03b2\u2010hydroxycortisol (6\u03b2\u2010OHF) and cortisol concentrations in DBS by liquid chromatography\/tandem mass spectrometry so as to utilize DBS as a less invasive blood sampling method for calculating the ratio of 6\u03b2\u2010OHF\/cortisol. The lower limits of quantification obtained using four DBS were 1.08\u2009pg\/50\u2009\u03bcl for 6\u03b2\u2010OHF and 1.01\u2009pg\/50\u2009\u03bcl for cortisol. The 6\u03b2\u2010OHF and cortisol in DBS were stable for 28\u2009days at room temperature. The intraday and interday accuracy and precision of the method was &lt;12%. Additionally, the 6\u03b2\u2010OHF and cortisol in DBS were measured before, during, and after 3\u2009days of clarithromycin administration to two of the subjects. Then, their concentration was compared in the plasma and whole blood collected simultaneously. The concentrations of 6\u03b2\u2010OHF and cortisol in four DBS ranged from 0.007 to 0.079\u2009ng\/50\u2009\u03bcl and from 1.15 to 6.66\u2009ng\/50\u2009\u03bcl, respectively. The 6\u03b2\u2010OHF\/cortisol ratio in DBS decreased by approximately 50% on administering clarithromycin compared with that before the administration of clarithromycin. The 6\u03b2\u2010OHF\/cortisol ratio in DBS also showed a strong correlation with that in whole blood (<jats:italic>r<\/jats:italic>\u2009=\u20090.9694) and plasma (<jats:italic>r<\/jats:italic>\u2009=\u20090.9383). This method provides high accuracy and precision for measuring 6\u03b2\u2010OHF and cortisol in DBS. It also allows the use of DBS instead of plasma for calculating the 6\u03b2\u2010OHF\/cortisol ratio. The 6\u03b2\u2010OHF\/cortisol ratio could be an index of CYP3A activity in clinical setting.<\/jats:p>","DOI":"10.1002\/jms.4790","type":"journal-article","created":{"date-parts":[[2021,11,2]],"date-time":"2021-11-02T17:47:16Z","timestamp":1635875236000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Dried blood spots analysis of 6\u03b2\u2010hydroxycortisol and cortisol using liquid chromatography\/tandem mass spectrometry for calculating 6\u03b2\u2010hydroxycortisol to cortisol ratio"],"prefix":"10.1002","volume":"56","author":[{"given":"Ryohei","family":"Hirano","sequence":"first","affiliation":[{"name":"Laboratory of Clinical Pharmacy and Experimental Therapeutics, School of Pharmacy Tokyo University of Pharmacy and Life Sciences  Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akitomo","family":"Yokokawa","sequence":"additional","affiliation":[{"name":"Laboratory of Clinical Pharmacy and Experimental Therapeutics, School of Pharmacy Tokyo University of Pharmacy and Life Sciences  Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tomomi","family":"Furihata","sequence":"additional","affiliation":[{"name":"Laboratory of Clinical Pharmacy and Experimental Therapeutics, School of Pharmacy Tokyo University of Pharmacy and Life Sciences  Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9062-7370","authenticated-orcid":false,"given":"Hiromi","family":"Shibasaki","sequence":"additional","affiliation":[{"name":"Laboratory of Clinical Pharmacy and Experimental Therapeutics, School of Pharmacy Tokyo University of Pharmacy and Life Sciences  Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,12,8]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1542\/peds.32.3.338"},{"issue":"4","key":"e_1_2_8_3_1","first-page":"288","article-title":"Advantages and challenges of dried blood spot analysis by mass spectrometry across the total testing process","volume":"27","author":"Zakaria R","year":"2016","journal-title":"eJIFCC"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/dta.1646"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-014-8415-2"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2014-1269"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0233724"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1097\/FTD.0000000000000673"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/bcpt.12801"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2014.10.040"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1742-7843.2012.00932.x"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/bcp.12544"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/clpt.2014.83"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40262-013-0115-0"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40262-019-00783-z"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1097\/00008571-199408000-00001"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02353475"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1124\/dmd.31.11.1283"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1124\/dmd.112.048561"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.steroids.2014.05.023"},{"key":"e_1_2_8_21_1","unstructured":"ShibasakiH FurutaT YokokawaA HiranoR.Method for evaluating CYP3A enzymatic activity in human body (phenotyping test method). 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