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Cellular proliferation and de novo synthesis of lipids are related to cancer progression. In this study, we evaluated the lipidomic profile of two\u2010dimensional (2D) monolayer and multicellular tumor spheroids from the HCT 116 colon carcinoma cell line. We utilized serial trypsinization on the spheroid samples to generate three cellular populations representing the proliferative, quiescent, and necrotic regions of the spheroid. This analysis enabled a comprehensive identification and quantification of lipids produced in each of the spheroid layer and 2D cultures. We show that lipid subclasses associated with lipid droplets form in oxygen\u2010restricted and acidic regions of spheroids and are produced at higher levels than in 2D cultures. Additionally, sphingolipid production, which is implicated in cell death and survival pathways, is higher in spheroids relative to 2D cells. Finally, we show that increased numbers of lipids composed of polyunsaturated fatty acids (PUFAs) are produced in the quiescent and necrotic regions of the spheroid. The lipidomic signature for each region and cell culture type highlights the importance of understanding the spatial aspects of cancer biology. These results provide additional lipid biomarkers in colon cancer cells that can be further studied to target pivotal lipid production pathways.<\/jats:p>","DOI":"10.1002\/jms.4880","type":"journal-article","created":{"date-parts":[[2022,8,8]],"date-time":"2022-08-08T00:29:01Z","timestamp":1659918541000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Lipidomic comparison of 2D and 3D colon cancer cell culture models"],"prefix":"10.1002","volume":"57","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4085-4188","authenticated-orcid":false,"given":"Fernando","family":"Tobias","sequence":"first","affiliation":[{"name":"Department of Chemistry and Biochemistry The Ohio State University  Columbus Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1969-9013","authenticated-orcid":false,"given":"Amanda B.","family":"Hummon","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry The Ohio State University  Columbus Ohio USA"},{"name":"Comprehensive Cancer Center The Ohio State University  Columbus Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,8,26]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21654"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21708"},{"issue":"1","key":"e_1_2_10_4_1","first-page":"113","article-title":"Growth of multicell spheroids in tissue culture as a model of nodular carcinomas","volume":"46","author":"Sutherland RM","year":"1971","journal-title":"J Natl Cancer Inst"},{"key":"e_1_2_10_5_1","first-page":"24","volume-title":"Growth and cellular characteristics of multicell spheroids. 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