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Sophisticated analytical techniques have proved valuable in the identification of human skin surface chemicals used by vector mosquitoes to find their human host. Polydimethylsiloxane (PDMS) was used as a non\u2010invasive passive wearable sampler to concentrate skin surface non\u2010volatile and semi\u2010volatile compounds prior to solvent desorption directly in an LC vial, thereby simplifying the link between extraction and analysis. Ultra\u2010performance liquid chromatography with ion mobility spectrometry coupled with high\u2010resolution mass spectrometry (UPLC\u2010IMS\u2010HRMS) was used for compound separation and detection. A comparison of the skin chemical profiles between the ankle and wrist skin surface region sampled over a 5\u2010day period for a human volunteer was done. Twenty\u2010three biomarkers were tentatively identified with the aid of a collision cross\u2010section (CCS) prediction tool, seven associated with the ankle skin surface region and 16 closely associated with the wrist skin surface. Ten amino acids were detected and unequivocally identified on the human skin surface for the first time. Furthermore, 22 previously unreported skin surface compounds were tentatively identified on the human skin surface using accurate mass, CCS values and fragmentation patterns. Method limits of detection for the passive skin sampling method ranged from 8.7 (sulfadimethoxine) to 95\u2009ng (taurine). This approach enabled the detection and identification of as\u2010yet unknown human skin surface compounds and provided corresponding CCS values.<\/jats:p>","DOI":"10.1002\/jms.4992","type":"journal-article","created":{"date-parts":[[2023,12,18]],"date-time":"2023-12-18T09:55:45Z","timestamp":1702893345000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Advancing the analytical toolkit in the investigation of vector mosquito host biting site selection"],"prefix":"10.1002","volume":"59","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3869-7467","authenticated-orcid":false,"given":"Madelien","family":"Wooding","sequence":"first","affiliation":[{"name":"Department of Chemistry University of Pretoria  Pretoria South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tyren","family":"Dodgen","sequence":"additional","affiliation":[{"name":"Waters Corporation  Rydalmere New South Wales 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