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By comparing mass spectrometry fingerprints, healthy leaves from those infected with powdery mildew and <jats:italic>Rhytisma acerinum<\/jats:italic> were distinguished. Leaves were grated with sandpaper, cut into triangles, and placed before the mass spectrometer, with the addition of a methanol\u2010formic acid solution. Multivariate statistical analysis categorized the samples into three groups. 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