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We carried out and validated an LC\u2013MS\/MS assay to quantify these compounds in rabbit tear fluid in order to look into the pharmacokinetics of these two drugs. We employed a protein precipitation technique for the extraction of drugs under examination. A Waters Symmetry C<jats:sub>18<\/jats:sub> column was used to separate the analytes and internal standard. The composition of the mobile phase was like (A) 0.1% v\/v formic acid in water and (B) methanol. The detection of MFX and AB was accomplished through the utilization of positive ion electrospray ionization under multiple reaction monitoring mode. The linearity curves for both analytes exhibited an acceptable trendline across a concentration range of 2.34\u2013300\u2009ng\/mL for MFX and 7.81\u20131000\u2009ng\/mL for AB in surrogate rabbit tear fluid. The lower limit of quantitation for MFX was 2.34\u2009ng\/mL, while for AB, it was 7.81\u2009ng\/mL. 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