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Vancomycin and meropenem are the two most commonly used antibiotics for local application. Determining the concentrations of vancomycin and meropenem in the serum and synovial fluid of patients with PJI plays a significant role in further optimizing local medication schemes and effectively eradicating biofilm infections. This study aimed to establish a rapid, sensitive, and accurate ultra\u2010performance liquid chromatography\u2013tandem mass spectrometry (UPLC\u2013MS\/MS) method for determining the concentrations of vancomycin and meropenem in human serum and synovial fluid. Serum samples were processed using acetonitrile precipitation of proteins and dichloromethane extraction, while synovial fluid samples were diluted before analysis. Chromatographic separation was achieved in 6\u00a0min on a Waters Acquity UPLC BEH C18 column, with the mobile phase consisting of 0.1% formic acid in water (solvent A) and acetonitrile (solvent B). Quantification was carried out using a Waters XEVO TQD triple quadrupole mass spectrometer with an electrospray ionization (ESI) source in positive ion mode. The multiple reaction monitoring (MRM) mode was employed to detect the following quantifier ion transitions: 717.95\u201399.97 (norvancomycin), 725.90\u2013100.04 (vancomycin), 384.16\u201367.99 (meropenem). The method validation conformed to the guidelines of the FDA and the Chinese Pharmacopoeia. The method demonstrated good linearity within the range of 0.5\u201350\u2009\u03bcg\/ml for serum and 0.5\u2013100\u2009\u03bcg\/ml for synovial fluid. Selectivity, intra\u2010day and inter\u2010day precision and accuracy, extraction recovery, matrix effect, and stability validation results all met the required standards. This method has been successfully applied in the pharmacokinetic\/pharmacodynamic (PK\/PD) studies of patients with PJI.<\/jats:p>","DOI":"10.1002\/jms.5041","type":"journal-article","created":{"date-parts":[[2024,5,16]],"date-time":"2024-05-16T02:55:07Z","timestamp":1715828107000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Determination of vancomycin and meropenem in serum and synovial fluid of patients with prosthetic joint infections using UPLC\u2013MS\/MS"],"prefix":"10.1002","volume":"59","author":[{"given":"Jiawei","family":"He","sequence":"first","affiliation":[{"name":"Department of Pharmacy The First Affiliated Hospital of Xinjiang Medical University  Urumqi China"},{"name":"Xinjiang Key Laboratory of Clinical Drug Research  Urumqi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Pharmacy The 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