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Topical fluoroquinolones combined with corticosteroids have been shown to be useful in the treatment of BK. A rapid, selective, and sensitive bioanalytical method for simultaneous quantification of Gatifloxacin (GAT) and Dexamethasone (DEX) has been developed and validated using tandem mass spectrometry (LC\u2013MS\/MS). Optimal separation was accomplished in under 5\u2009min using an Agilent Zorbax C18 column (100\u2009mm\u2009\u00d7\u20094.6\u2009mm, 3.5\u2009\u03bcm). The mobile phase was composed of a blend of 0.2% formic acid in triple distilled water and methanol with a flow rate of 0.65\u2009mL\/min in isocratic mode. GAT and DEX were detected in positive electrospray ionization multiple reaction monitoring mode (MRM), and the retention time was found to be at 1.64 and 2.93\u2009min, respectively. The linearity of GAT and DEX was found to be in the range of 1.56\u2013400\u2009ng\u2009mL<jats:sup>\u22121<\/jats:sup> with good precision and accuracy. The method was validated according to USFDA regulatory guidelines. The validated method was effectively utilized for preclinical pharmacokinetic analysis of GAT and DEX in rabbit tear fluid following the topical application of a commercial formulation.<\/jats:p>","DOI":"10.1002\/jms.5088","type":"journal-article","created":{"date-parts":[[2024,10,1]],"date-time":"2024-10-01T04:13:43Z","timestamp":1727756023000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Pharmacokinetic Analysis of Gatifloxacin and Dexamethasone in Rabbit Ocular Biofluid Using a Sensitive and Selective LC\u2013MS\/MS Method"],"prefix":"10.1002","volume":"59","author":[{"given":"Arpon","family":"Biswas","sequence":"first","affiliation":[{"name":"Pharmaceutics and Pharmacokinetic Division CSIR\u2010Central Drug Research Institute  Lucknow India"},{"name":"Pharmaceutics and Pharmacokinetics Division of CSIR\u2010Central Drug Research Institute Jawaharlal Nehru University  New Delhi 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