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This study investigates the antidiabetic potential of <jats:italic>Cassia bakeriana<\/jats:italic> bark extracts and fractions. We evaluate their ability to inhibit \u03b1\u2010amylase and \u03b1\u2010glucosidase enzymes and advanced glycation end\u2010products (AGEs). The antioxidant potential was also examined. Extracts were prepared through maceration with hexane (HE) and ethanol (EE), and the fractions were obtained via liquid\u2013liquid extraction from EE. Anti\u2010enzymatic, anti\u2010glycation, antioxidant, and cytotoxic assays were conducted in 96\u2010well plates using different concentrations of samples to determine the half\u2010maximal inhibitory concentration (IC<jats:sub>50<\/jats:sub>). Active samples were further analyzed using HPLC\u2010(\u2212)\u2010ESI\u2010MS\/MS. The ethyl acetate fraction (EAF) demonstrated a high percentage of \u03b1\u2010amylase inhibition (94.0%) with a promising IC<jats:sub>50<\/jats:sub> value of 1.05\u2009\u03bcg\u2009mL<jats:sup>\u22121<\/jats:sup>. Additionally, EAF displayed 61.5% inhibition of \u03b1\u2010glucosidase, with an IC<jats:sub>50<\/jats:sub> value of 537\u2009\u03bcg\u2009mL<jats:sup>\u22121<\/jats:sup>. The EE, EAF, and <jats:italic>n<\/jats:italic>\u2010butanol fraction (BF) exhibited strong anti\u2010glycation capacities. Furthermore, the EE, EAF, BF, and dichloromethane fractions showed promising antioxidant activity using the DPPH and ORAC methodologies. Cytotoxic activity was also evaluated with Vero cells, revealing no adverse effects on cell viability (CC<jats:sub>50<\/jats:sub>\u2009&gt;\u2009512.0\u2009\u03bcg\u2009mL<jats:sup>\u22121<\/jats:sup>). Active samples predominantly comprised proanthocyanidins, flavonoids, and anthraquinone, representing the main constituents of C. <jats:italic>bakeriana<\/jats:italic> bark. This study provides the first assessment of the antidiabetic potential of <jats:italic>C. bakeriana<\/jats:italic> bark and a comprehensive analysis of the chemical composition of its active extracts and fractions, offering hope for future treatments.<\/jats:p>","DOI":"10.1002\/jms.5099","type":"journal-article","created":{"date-parts":[[2024,11,15]],"date-time":"2024-11-15T03:52:41Z","timestamp":1731642761000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Phytochemical Profiling by HPLC\u2010ESI\u2010MS\/MS and In\u00a0Vitro Investigation of the Antidiabetic Activity of <i>Cassia bakeriana<\/i> Bark Extract and Fractions"],"prefix":"10.1002","volume":"59","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7192-7765","authenticated-orcid":false,"given":"Diego\u00a0G.","family":"Prado","sequence":"first","affiliation":[{"name":"Nucleus of Research in Natural Products, Institute of Chemistry Federal University of 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