{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,17]],"date-time":"2025-10-17T00:32:25Z","timestamp":1760661145865,"version":"build-2065373602"},"reference-count":17,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,1,5]],"date-time":"2025-01-05T00:00:00Z","timestamp":1736035200000},"content-version":"vor","delay-in-days":4,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Mass Spectrom"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>In our previous work, the sodiation of melittin, cytochrome <jats:italic>c<\/jats:italic>, and ubiquitin in a 1\u2009mM NaOH water\/methanol solution was studied by electrospray mass spectrometry. It was suggested that the \u03b1\u2010helix is more resistant to sodiation than the \u03b2\u2010sheet. In this study, sodiation of enhanced green fluorescent protein (EGFP) composed of a \u03b2\u2010barrel was studied in 1% CH<jats:sub>3<\/jats:sub>COOH (AcOH) or 1\u2009mM NaOH water\/methanol solution by electrospray mass spectrometry. Although EGFP was denatured in an acidic solution, it maintains a near\u2010native structure in a basic solution. For the 1% AcOH solution, the protonated EGFP, [EGFP\u2009+\u2009nH\u2009\u2212\u2009mH\u2009+\u2009mNa]<jats:sup>n+<\/jats:sup>, with <jats:italic>n<\/jats:italic>\u2009=\u200914\u2009\u2212\u200936 and <jats:italic>m<\/jats:italic>\u2009=\u20090 was detected. For 1\u2009mM NaOH, the number <jats:italic>n<\/jats:italic> for [EGFP\u2009+\u2009nH\u2009\u2212\u2009mH\u2009+\u2009mNa]<jats:sup>n+<\/jats:sup> was found to increase with the sodiation number <jats:italic>m<\/jats:italic> and vice versa for [EGFP\u2009+\u2009nH\u2009\u2212\u2009mH\u2009+\u2009mNa]<jats:sup>n\u2212<\/jats:sup>. Namely, Na<jats:sup>+<\/jats:sup> adducts counteract the negative charges of deprotonated acidic residues. The protonated EGFP detected as major ions for basic 1\u2009mM NaOH was ascribed to the more surface\u2010active H<jats:sub>3<\/jats:sub>O<jats:sup>+<\/jats:sup>(aq) than OH<jats:sup>\u2212<\/jats:sup>(aq).<\/jats:p>","DOI":"10.1002\/jms.5111","type":"journal-article","created":{"date-parts":[[2025,1,5]],"date-time":"2025-01-05T22:54:28Z","timestamp":1736117668000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Sodiation of Enhanced Green Fluorescent Protein (EGFP) in Basic Solution Studied by Electrospray Mass Spectrometry"],"prefix":"10.1002","volume":"60","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5352-5914","authenticated-orcid":false,"given":"Kenzo","family":"Hiraoka","sequence":"first","affiliation":[{"name":"Clean Energy Research Center University of Yamanashi  Kofu Yamanashi Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Satoshi","family":"Ninomiya","sequence":"additional","affiliation":[{"name":"Clean Energy Research Center University of Yamanashi  Kofu Yamanashi Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephanie","family":"Rankin\u2010Turner","sequence":"additional","affiliation":[{"name":"Department of Chemistry University of Pittsburgh  Pittsburgh Pennsylvania USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Noa","family":"Suzuki","sequence":"additional","affiliation":[{"name":"Graduate School of Medical Life Science Yokohama City University  Yokohama Kanagawa Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Satoko","family":"Akashi","sequence":"additional","affiliation":[{"name":"Graduate School of Medical Life Science Yokohama City University  Yokohama Kanagawa Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,1,5]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1030590302"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1039\/b913033k"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-008-2515-9"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biochem.67.1.509"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2020.08.224"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-407699-0.00004-2"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja0552693"},{"issue":"1","key":"e_1_2_7_9_1","first-page":"1","article-title":"Gas\u2010Phase Ion and Neutral Thermochemistry","volume":"17","author":"Lias S. 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