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This study explores the antidiabetic potential of 25% aqueous methanol extract of <jats:italic>Cupressus torulosa<\/jats:italic> needles, utilizing in\u00a0vitro and in\u00a0vivo assays. In the <jats:italic>\u03b1<\/jats:italic>\u2010glucosidase inhibition assay, the extract exhibited significant in\u00a0vitro antidiabetic activity with an IC<jats:sub>50<\/jats:sub>, 123.45\u2009\u00b1\u20091.8\u2009\u03bcg\/mL, comparable to the standard drug acarbose (IC<jats:sub>50<\/jats:sub>, 58.21\u2009\u00b1\u20093.1\u2009\u03bcg\/mL). Toxicity assessment indicated non\u2010toxic nature of the extract at 2000\u2009mg\/kg b.w. In STZ\u2010induced diabetic mice, it displayed dose\u2010dependent antihyperglycemic effects, evident at the fourth hour and 14th day, paralleling the positive control glibenclamide. In the chemical profiling of the extract using UPLC\u2010QTOF\u2010MS, the mobile phases consisted of 0.1% formic acid in water (Solvent A) and 100% methanol (Solvent B). The gradient elution started with 5% B (0 to 2\u2009min) and gradually increased to 95% B by 25\u2009min, followed by a post\u2010run time of 2\u2009min. Preliminarily, 50 constituents were identified, predominantly phenolics, with hypoglycemic effects attributed to flavonoids like (\u2212)\u2010epicatechin, amentoflavone, and cupressuflavone, as well as iridoid <jats:italic>O<\/jats:italic>\u2010glycoside, exemplified by haprpagoside. Further studies are needed to assess the long\u2010term efficacy, safety, and molecular mechanisms of the extract of <jats:italic>C. torulosa<\/jats:italic> needles in diabetes management.<\/jats:p>","DOI":"10.1002\/jms.5117","type":"journal-article","created":{"date-parts":[[2025,2,26]],"date-time":"2025-02-26T01:00:27Z","timestamp":1740531627000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["In Vitro and In\u00a0Vivo Assessment of Antidiabetic Activity of <i>Cupressus torulosa<\/i> D. Don Needles, Their LCQTOFMS Assisted Metabolite Profiling, and Implications for Diabetes Management"],"prefix":"10.1002","volume":"60","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2887-6595","authenticated-orcid":false,"given":"Radhika","family":"Khanna","sequence":"first","affiliation":[{"name":"Chemistry &amp; Bioprospecting Division Forest Research Institute  Dehradun India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.\u00a0R.","family":"Chitme","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy Dehradun Institute of Technology University  Dehradun India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Khushaboo","family":"Bhadoriya","sequence":"additional","affiliation":[{"name":"Chemistry &amp; Bioprospecting Division Forest Research Institute  Dehradun India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y.\u00a0C.","family":"Tripathi","sequence":"additional","affiliation":[{"name":"Chemistry &amp; 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