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Flash chromatography and thin layer chromatography\u2013mass spectrometry (TLC\u2013MS) are powerful analytical tools that play a crucial role in the purification, identification, and quality control of herbal products. Flash chromatography enables rapid separation and purification of bioactive compounds from complex herbal mixtures, while thin layer chromatography provides a simple and effective method for compound fingerprinting. When coupled with mass spectrometry, thin layer chromatography\u2013mass spectrometry offers highly sensitive and precise identification of individual compounds, facilitating the detection of active ingredients, metabolites, and contaminants. This article explores the principles, techniques, and applications of flash chromatography and thin layer chromatography\u2013mass spectrometry in the analysis of herbal formulations, with a focus on their combined use for the purification and characterization of active constituents. Additionally, it highlights the importance of these methods in quality control, standardization, and regulatory compliance in the herbal industry. Flash chromatography offers a distinct advantage in the rapid and high\u2010resolution separation of complex multi\u2010component herbal matrices, enabling efficient recovery of target bioactives, whereas TLC\u2013MS provides exceptional analytical sensitivity and specificity for the detection and structural elucidation of low\u2010abundance constituents. The combined application of these techniques ensures comprehensive chemical profiling and robust quality control of herbal formulations. By integrating these advanced techniques, researchers and manufacturers can ensure the integrity, potency, and safety of herbal products, paving the way for more effective and reliable herbal medicine.<\/jats:p>","DOI":"10.1002\/jms.5172","type":"journal-article","created":{"date-parts":[[2025,8,30]],"date-time":"2025-08-30T06:35:47Z","timestamp":1756535747000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Enhancing Herbal Formulation Analysis: The Role of Flash Chromatography and TLC\u2013MS in Purification and Quality Control"],"prefix":"10.1002","volume":"60","author":[{"given":"Saurabh","family":"Shukla","sequence":"first","affiliation":[{"name":"Nootan Pharmacy College Sankalchand Patel University  Visnagar Gujarat India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2512-2536","authenticated-orcid":false,"given":"Shweta","family":"Mevada","sequence":"additional","affiliation":[{"name":"Nootan Pharmacy College Sankalchand Patel University  Visnagar Gujarat India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,8,29]]},"reference":[{"key":"e_1_2_14_2_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2023.1265178"},{"key":"e_1_2_14_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.sajb.2020.06.031"},{"key":"e_1_2_14_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules28041845"},{"key":"e_1_2_14_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms24032019"},{"key":"e_1_2_14_6_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmedt.2024.1358956"},{"key":"e_1_2_14_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.hermed.2024.100916"},{"key":"e_1_2_14_8_1","doi-asserted-by":"publisher","DOI":"10.14218\/FIM.2023.00086"},{"key":"e_1_2_14_9_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2013.00177"},{"key":"e_1_2_14_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2020.113477"},{"key":"e_1_2_14_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fitote.2010.08.013"},{"key":"e_1_2_14_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2020.128115"},{"key":"e_1_2_14_13_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsomega.9b03060"},{"issue":"2","key":"e_1_2_14_14_1","first-page":"156","article-title":"Separation Techniques: Chromatography","volume":"3","author":"Coskun O.","year":"2016","journal-title":"Northern Clinics of Istanbul"},{"key":"e_1_2_14_15_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13020-018-0177-x"},{"key":"e_1_2_14_16_1","doi-asserted-by":"publisher","DOI":"10.4314\/ajtcam.v8i1.60483"},{"key":"e_1_2_14_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcoa.2024.100131"},{"key":"e_1_2_14_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11030\u2010008\u20109104\u2010x"},{"key":"e_1_2_14_19_1","doi-asserted-by":"publisher","DOI":"10.1201\/b12527-22"},{"key":"e_1_2_14_20_1","doi-asserted-by":"publisher","DOI":"10.3389\/fchem.2022.800729"},{"key":"e_1_2_14_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcoa.2021.100023"},{"key":"e_1_2_14_22_1","doi-asserted-by":"publisher","DOI":"10.31083\/j.fbe1604035"},{"issue":"9","key":"e_1_2_14_23_1","article-title":"Echinacea purpurea (L.) 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