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However, a comprehensive investigation into its pharmacodynamic material basis has not been conducted. This study presents a method that integrates UHPLC\u2010HRMS and UHPLC\u2013MS\/HRMS with network pharmacology to investigate the pharmacodynamic components, multi\u2010tissue distribution and biotransformation processes of BYQD. This study systematically identified 20 pharmacodynamic constituents in plasma following BYQD administration and characterized their distribution patterns across six tissues (heart, liver, spleen, lung, kidney, and nasopharynx). Through integrated network pharmacology and molecular docking validation, four critical bioactive components were prioritized: quercetin, jaceosidin, \u03b1\u2010allocryptopine, and magnoflorine. Furthermore, we established the first comprehensive multi\u2010tissue metabolic atlas for BYQD, identifying 40 phase I\/II metabolites with tissue\u2010specific metabolic signatures. These findings delineate the in\u00a0vivo process of BYQD components, unravel their pharmacodynamic material basis.<\/jats:p>","DOI":"10.1002\/jms.5179","type":"journal-article","created":{"date-parts":[[2025,9,25]],"date-time":"2025-09-25T11:52:59Z","timestamp":1758801179000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Deciphering the Pharmacodynamic Basis of Biyan Qingdu Granules: Integrated key Pharmacodynamic Components Identification, Multi\u2010Tissue Distribution, and Metabolic Profiling"],"prefix":"10.1002","volume":"60","author":[{"given":"Na","family":"Zhang","sequence":"first","affiliation":[{"name":"School of Pharmacy Shenyang Pharmaceutical University  Shenyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiang","family":"Fan","sequence":"additional","affiliation":[{"name":"School of Traditional Chinese Medicine Beijing University of Chinese Medicine  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fengyi","family":"Lv","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Research Center for Marine Drugs, Renji Hospital, School of Medicine Shanghai JiaoTong University  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peng","family":"Li","sequence":"additional","affiliation":[{"name":"Hutchison Whampoa Guangzhou Baiyunshan Chinese Medicine co., ltd.  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