{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T03:38:41Z","timestamp":1779334721564,"version":"3.51.4"},"reference-count":47,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2025,10,24]],"date-time":"2025-10-24T00:00:00Z","timestamp":1761264000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Mass Spectrom"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Microsampling techniques such as dried blood spot (DBS) and dried blood matrix (DBM) offer several advantages over venipuncture sampling techniques, including ease of collection, reduced sample volume, improved sample stability and simplified transportation. In this study, mass spectrometry, a highly sensitive analytical instrument capable of handling small sample volumes, was used to compare venipuncture, DBS and DBM sampling methods for the detection of haemoglobin variants\u2014HbS, HbE and HbD\u2010Punjab\u2014and thalassemia in adults. Anonymised leftover K2\u2010EDTA venipuncture blood samples were used, including 10 samples each of normal, HbS trait\/disease, HbE trait, HbD trait and \u03b1\u2010thalassemia (HbH), as well as eight samples of \u03b2\u2010thalassemia. Three biomarker ratios\u2014S\/\u03b2 for HbS, \u03b1\/\u03b2 for \u03b1\u2010thalassemia (HbH) and \u03b4\/\u03b2 (%) for \u03b2\u2010thalassemia\u2014were analyzed. All the sampling techniques successfully distinguished sickle cell trait from sickle cell disease, \u03b2\u2010thalassemia trait from \u03b2\u2010thalassemia major and detected HbE and HbD\u2010Punjab traits. There were no statistically significant differences between sampling techniques in the qualitative detection of HbS and HbD\u2010Punjab variants. However, HbE variant, \u03b1\/\u03b2 and \u03b4\/\u03b2 (%) values were altered in DBS and DBM when compared with venipuncture blood. Statistical evaluation necessitates the need for matrix\u2010matched reference ranges for quantitative thalassemia analysis. Triple quadrupole mass spectrometry provides a unified platform for analysing blood samples using diverse sampling techniques, minimising logistical challenges and enabling efficient, accurate high\u2010throughput screening for haemoglobinopathies in clinical, population\u2010wide and resource\u2010limited settings.<\/jats:p>","DOI":"10.1002\/jms.5186","type":"journal-article","created":{"date-parts":[[2025,10,24]],"date-time":"2025-10-24T08:37:32Z","timestamp":1761295052000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Evaluating Dried Blood Spot and Dried Blood Matrix as Alternatives to Venipuncture for Diagnosing Haemoglobinopathies in Adults: A Mass Spectrometric Approach"],"prefix":"10.1002","volume":"60","author":[{"given":"Ankitha\u00a0K.","family":"Puthiyaveettil","sequence":"first","affiliation":[{"name":"R&amp;D Neuberg Anand Academy of Laboratory Medicine Pvt Ltd  Bengaluru 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