{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T16:50:47Z","timestamp":1790095847480,"version":"4.0.1"},"reference-count":16,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T00:00:00Z","timestamp":1759881600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Mass Spectrom"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Steroidal esters (e.g., testosterone, nandrolone and boldenone esters) belong to the substance class prohibited in professional sport that is particularly frequently abused due to its considerable performance\u2010enhancing effects. Thus, a high number of adverse analytical findings in doping controls is reported every year. Unfortunately, the analysis of steroids that are not exclusively of exogenous nature and, in particular, their differentiation from endogenously produced steroids is very time\u2010consuming and resource\u2010intensive (necessitating isotope\u2010ratio mass spectrometric analysis). The direct detection of the applied (unequivocally exogenous) steroidal esters in blood or dried blood spots (DBS) can offer a much simpler approach. In the present project, the analysis of 17 underivatized steroidal esters (composed of testosterone, nandrolone and boldenone species) is reported, employing liquid chromatography\u2010(low\u2010resolution)\u2010MS\n                    <jats:sup>n<\/jats:sup>\n                    . The results show that all included esters can be selectively and sensitively detected in MS\n                    <jats:sup>3<\/jats:sup>\n                    mode at sub\u2010ng\/mL levels in DBS. The detection limits for most analytes extracted from a single spot were below 0.1\u2009ng\/mL, and recovery rates were determined at 40%\u201380%. The overall procedure was controlled using four different stable isotope\u2010labelled internal standards. Notably, the obtained results are largely independent from the sampling device, and the method works for cellulose\u2010based DMPK cards as well as polymer\u2010based TASSO devices. Proof\u2010of\u2010concept and applicability of the method to authentic samples were demonstrated by analysing post\u2010administration samples collected after an oral administration of 80\u2009mg of testosterone undecanoate.\n                  <\/jats:p>","DOI":"10.1002\/jms.5188","type":"journal-article","created":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T21:24:30Z","timestamp":1759958670000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Simplified Analysis of Native Steroid Esters in Dried Blood Spots by LC\u2013MS\n                    <sup>3<\/sup>"],"prefix":"10.1002","volume":"60","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1199-0743","authenticated-orcid":false,"given":"Andreas","family":"Thomas","sequence":"first","affiliation":[{"name":"Institute of Biochemistry\/Center for Preventive Doping Research German Sport University Cologne  Cologne Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jasmin","family":"Thelen","sequence":"additional","affiliation":[{"name":"Institute of Biochemistry\/Center for Preventive Doping Research German Sport University Cologne  Cologne Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Panagiotis","family":"Sakellariou","sequence":"additional","affiliation":[{"name":"Institute of Biochemistry\/Center for Preventive Doping Research German Sport University Cologne  Cologne Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1535-6451","authenticated-orcid":false,"given":"Mario","family":"Thevis","sequence":"additional","affiliation":[{"name":"Institute of Biochemistry\/Center for Preventive Doping Research German Sport University Cologne  Cologne Germany"},{"name":"European Monitoring Center for Emerging Doping Agents (EuMoCEDA)  Cologne\/Bonn Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,10,8]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1080\/10408363.2022.2103085"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/dta.1560"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/dta.1914"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2014.03.013"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2020.113807"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/dta.3638"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/bmc.5633"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/dta.3226"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/dta.3889"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/dta.3681"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/dta.3681"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2022.123535"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/dta.3030"},{"key":"e_1_2_8_15_1","unstructured":"\u201chttps:\/\/www.wada\u2010ama.org\/sites\/default\/files\/resources\/files\/isl_2021.pdf\u201d in 2021."},{"key":"e_1_2_8_16_1","unstructured":"WADA \u201chttps:\/\/www.wada\u2010ama.org\/sites\/default\/files\/2023\u201002\/td2023idcrv1.1_eng_final.pdf\u201d in 2023."},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.3c05576"}],"container-title":["Journal of Mass Spectrometry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jms.5188","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T19:11:57Z","timestamp":1762283517000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/jms.5188"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,10,8]]},"references-count":16,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2025,11]]}},"alternative-id":["10.1002\/jms.5188"],"URL":"https:\/\/doi.org\/10.1002\/jms.5188","archive":["Portico"],"relation":{},"ISSN":["1076-5174","1096-9888"],"issn-type":[{"value":"1076-5174","type":"print"},{"value":"1096-9888","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,10,8]]},"assertion":[{"value":"2025-08-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-09-24","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-10-08","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e5188"}}