{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T22:10:08Z","timestamp":1783116608300,"version":"3.54.6"},"reference-count":42,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T00:00:00Z","timestamp":1759968000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Mass Spectrom"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    In this study, we present the first comprehensive application of ion mobility mass spectrometry (IMS MS) combined with collision\u2010induced dissociation (CID MS\/MS) to the comparative analysis of the gangliosidome in human hippocampal tissue affected by temporal lobe epilepsy and corresponding healthy controls. Using nanoESI IMS MS, we profiled complex ganglioside mixtures extracted from the human hippocampus affected by temporal lobe epilepsy and the normal hippocampus. A total of 217 ions corresponding to 192 distinct ganglioside species were identified in the epileptic tissue, compared with 156 ions assigned to 137 species in the healthy hippocampus. The majority of these species were polysialylated and exhibited extensive structural diversity in both glycan and ceramide moieties, with important modifications including fucosylation,\n                    <jats:italic>O<\/jats:italic>\n                    \u2010acetylation, GalNAc, and CH\n                    <jats:sub>3<\/jats:sub>\n                    COO\n                    <jats:sup>\u2212<\/jats:sup>\n                    attachments. The gangliosidome associated with epilepsy was found characterized by a higher overall degree of sialylation than previously known, with the exclusive presence of highly sialylated GP, GS, and GO species reported here for the first time in relation to this disease. CID MS\/MS experiments enabled the structural elucidation of several biologically relevant species, including\n                    <jats:italic>O<\/jats:italic>\n                    \u2010Ac\u2013GD1b (d18:1\/20:2) and GT1b (d18:1\/23:0), demonstrating the method's capacity to resolve complex structures and identify specific ganglioside isomers. Significant differences in the expression and modification patterns between pathological and control samples suggest disease\u2010associated remodeling of membrane components. This study not only reveals novel molecular features of epilepsy\u2010related ganglioside alterations but also establishes IMS CID MS\/MS as a powerful analytical platform for advancing glycosphingolipidomics and exploring biomolecular signatures in neurological disorders.\n                  <\/jats:p>","DOI":"10.1002\/jms.5190","type":"journal-article","created":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T10:56:32Z","timestamp":1760007392000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Advanced Ganglioside Characterization in Epileptic Human Hippocampus by Travelling Waves Ion Mobility Tandem Mass Spectrometry"],"prefix":"10.1002","volume":"60","author":[{"given":"Maria\u2010Roxana","family":"Biricioiu","sequence":"first","affiliation":[{"name":"National Institute for Research and Development in Electrochemistry and Condensed Matter  Timisoara Romania"},{"name":"Department of Physics West University of Timisoara  Timisoara Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4309-5447","authenticated-orcid":false,"given":"Kristina","family":"Mlinac\u2010Jerkovi\u0107","sequence":"additional","affiliation":[{"name":"Croatian Institute for Brain Research, School of Medicine University of Zagreb  Zagreb Croatia"},{"name":"Department of Chemistry and Biochemistry, School of Medicine University of Zagreb  Zagreb Croatia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katarina","family":"Ilic","sequence":"additional","affiliation":[{"name":"Croatian Institute for Brain Research, School of Medicine University of Zagreb  Zagreb Croatia"},{"name":"BRAIN Centre, Department of Neuroimaging, Institute of Psychiatry, Psychology and Neuroscience (IOPPN) King's College London  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomislav","family":"Sajko","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery University Hospital Sestre Milosrdnice  Zagreb Croatia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raluca","family":"Ica","sequence":"additional","affiliation":[{"name":"National Institute for Research and Development in Electrochemistry and Condensed Matter  Timisoara Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mirela","family":"Sarbu","sequence":"additional","affiliation":[{"name":"National Institute for Research and Development in Electrochemistry and Condensed Matter  Timisoara Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David\u00a0E.","family":"Clemmer","sequence":"additional","affiliation":[{"name":"Department of Chemistry, The College of Arts &amp; Science Indiana University  Bloomington Indiana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Svjetlana","family":"Kalanj\u2010Bognar","sequence":"additional","affiliation":[{"name":"Croatian Institute for Brain Research, School of Medicine University of Zagreb  Zagreb Croatia"},{"name":"Department of Chemistry and Biochemistry, School of Medicine University of Zagreb  Zagreb Croatia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alina\u00a0D.","family":"Zamfir","sequence":"additional","affiliation":[{"name":"National Institute for Research and Development in Electrochemistry and Condensed Matter  Timisoara Romania"},{"name":"Department of Technical and Natural Sciences Aurel Vlaicu University of Arad  Arad Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,10,9]]},"reference":[{"key":"e_1_2_8_2_1","volume-title":"Handbook of Clinical Neurology","author":"Aminoff J. M.","year":"2022"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.carj.2013.05.001"},{"key":"e_1_2_8_4_1","volume-title":"Encyclopedia of Clinical Neuropsychology","author":"Kreutzer J. S.","year":"2018"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.sult.2007.09.003"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00276-015-1582-9"},{"key":"e_1_2_8_7_1","first-page":"58","article-title":"Temporal Lobe Epilepsy With Hippocampus Sclerosis: Prevalence, Etiology, and Treatment Options With Herbal Medicines","volume":"17","author":"Wasim M.","year":"2022","journal-title":"Pakistan Journal of Neurological Sciences"},{"key":"e_1_2_8_8_1","first-page":"1","article-title":"Association of Epilepsy and Neurological Impairments With Homozygous Recessive Missense Mutations Found in the Genes Responsible for Ganglioside Biosynthesis (ST3GAL5) and Calcium Voltage\u2010Gated Channels (CACNA1H)\u2014Insights Through Molecular Dynamic Simulations","volume":"1","author":"Abid R.","year":"2024","journal-title":"Journal of Biomolecular Structure & Dynamics"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.crvi.2004.10.010"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.0000000000007447"},{"key":"e_1_2_8_11_1","article-title":"Start Me Up: How Can Surrounding Gangliosides Affect Sodium\u2010Potassium ATPase Activity and Steer Towards Pathological Ion Imbalance in Neurons?","volume":"10","author":"Puljko B.","year":"2022","journal-title":"Biomedicine"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1212\/01.wnl.0000187129.66353.13"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1155\/2012\/630853"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.1992.tb09782.x"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1535-7511.2005.00059.x"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41423-020-0388-9"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnins.2020.572965"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnins.2024.1391413"},{"key":"e_1_2_8_19_1","first-page":"107","article-title":"Ganglioside Changes Associated With Temporal Lobe Epilepsy in the Human Hippocampus","volume":"60","author":"Yu R. K.","year":"1987","journal-title":"Yale Journal of Biology and Medicine"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1021\/jasms.3c00360"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms25021335"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules27134056"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/bs.accb.2018.09.002"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.6b00155"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1039\/C8AN01118D"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biochi.2017.05.016"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1021\/jasms.1c00088"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biochi.2020.08.011"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biochi.2025.01.011"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1515\/BC.2001.033"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2760(80)90227-1"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.carres.2013.09.007"},{"key":"e_1_2_8_33_1","first-page":"348","volume-title":"Methods in Enzymology","author":"Schnaar R. L.","year":"1994"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00906a015"},{"key":"e_1_2_8_35_1","first-page":"11","volume-title":"Advances in Experimental Medicine and Biology","author":"Svennerholm L.","year":"1980"},{"key":"e_1_2_8_36_1","first-page":"293","article-title":"IUPAC\u2010IUB Joint Commission on Biochemical Nomenclature (JCBN)","volume":"257","author":"Chester M. A.","year":"1998","journal-title":"European Journal of Biochemistry"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01049915"},{"key":"e_1_2_8_38_1","first-page":"45","volume-title":"Progress in Brain Research","author":"Costello C. E.","year":"1994"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/1044-0305(92)87010-V"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.2478\/v10134-010-0043-6"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ab.2017.01.010"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pbb.2009.09.015"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1093\/glycob\/cws103"}],"container-title":["Journal of Mass Spectrometry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jms.5190","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T19:12:00Z","timestamp":1762283520000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/jms.5190"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,10,9]]},"references-count":42,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2025,11]]}},"alternative-id":["10.1002\/jms.5190"],"URL":"https:\/\/doi.org\/10.1002\/jms.5190","archive":["Portico"],"relation":{},"ISSN":["1076-5174","1096-9888"],"issn-type":[{"value":"1076-5174","type":"print"},{"value":"1096-9888","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,10,9]]},"assertion":[{"value":"2025-07-24","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-09-24","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-10-09","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e5190"}}