{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T17:16:15Z","timestamp":1790356575290,"version":"4.1.0"},"reference-count":30,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2004,2,18]],"date-time":"2004-02-18T00:00:00Z","timestamp":1077062400000},"content-version":"vor","delay-in-days":17,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Mass Spectrom."],"published-print":{"date-parts":[[2004,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Antimicrobial peptides (AMPs), named lycocitin 1, 2 and 3, and a peptide with a monoisotopic molecular mass of 3038.70 Da were detected in the venom glands of the wolf spider <jats:italic>Lycosa singoriensis<\/jats:italic>. Two of the peptides, lycocitin 1 and 2, are new AMPs whereas lycocitin 3 is highly homologous to lycotoxin II isolated from the venom of spider <jats:italic>Lycosa carolinensis<\/jats:italic>. In addition, two other peptides with monoisotopic masses of 2034.20 and 2340.28 Da showing the motif typical for antimicrobial peptides were also identified. These peptides and lycocitin 1, 2 and 3 were <jats:italic>de novo<\/jats:italic> sequenced using electron capture dissociation and low\u2010energy collisional tandem mass spectrometry. The amino acid sequence of lycocitin 1 was determined as GKLQAFLAKMKEIAAQTL\u2010NH<jats:sub>2<\/jats:sub>. Lycocitin 2 differs from lycocitin 1 by a replacement of a lysine residue for an arginine residue at the second position. Lycocitin 3 differs from the known lycotoxin II consisting of 27 amino acid residues by a deletion of Gly\u201026. Both lycocitin 1 and 2 inhibit growth of Gram\u2010positive (<jats:italic>Staphylococcus aureus, Bacillus subtilis<\/jats:italic>) and Gram\u2010negative (<jats:italic>Escherichia coli<\/jats:italic>) bacteria and fungi (<jats:italic>Candida albicans, Pseudomonas aeruginosa<\/jats:italic>) at micromolar concentrations. Copyright \u00a9 2004 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/jms.577","type":"journal-article","created":{"date-parts":[[2004,2,26]],"date-time":"2004-02-26T15:23:56Z","timestamp":1077809036000},"page":"193-201","source":"Crossref","is-referenced-by-count":62,"title":["<i>De novo<\/i> sequencing of antimicrobial peptides isolated from the venom glands of the wolf spider <i>Lycosa singoriensis<\/i>"],"prefix":"10.1002","volume":"39","author":[{"given":"B. A.","family":"Budnik","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. 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