{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,14]],"date-time":"2026-05-14T16:38:28Z","timestamp":1778776708496,"version":"3.51.4"},"reference-count":14,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2004,6,8]],"date-time":"2004-06-08T00:00:00Z","timestamp":1086652800000},"content-version":"vor","delay-in-days":7,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Mass Spectrom."],"published-print":{"date-parts":[[2004,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In the pharmaceutical industry, a higher attrition rate during the drug discovery process means a lower drug failure rate in the later stages. This translates into shorter drug development time and reduced cost for bringing a drug to market. Over the past few years, analytical strategies based on liquid chromatography\/mass spectrometry (LC\/MS) have gone through revolutionary changes and presently accommodate most of the needs of the pharmaceutical industry. Among these LC\/MS techniques, collision induced dissociation (CID) or tandem mass spectrometry (MS\/MS and MS<jats:sup><jats:italic>n<\/jats:italic><\/jats:sup>) techniques have been widely used to identify unknown compounds and characterize metabolites. MS\/MS methods are generally ineffective for distinguishing isomeric compounds such as metabolites involving oxygenation of carbon or nitrogen atoms. Most recently, atmospheric pressure ionization (API) source decomposition methods have been shown to aid in the mass spectral distinction of isomeric oxygenated (<jats:italic>N<\/jats:italic>\u2010oxide vs hydroxyl) products\/metabolites. In previous studies, experiments were conducted using mass spectrometers equipped with a heated capillary interface between the mass analyzer and the ionization source. In the present study, we investigated the impact of the length of a heated capillary or heated ion transfer tube (a newer version of the heated capillary designed for accommodating orthogonal API source design) in inducing for\u2010API source deoxygenation that allows the distinction of <jats:italic>N<\/jats:italic>\u2010oxide from hydroxyl compounds. 8\u2010Hydroxyquinoline (HO\u2010Q), quinoline\u2010<jats:italic>N<\/jats:italic>\u2010oxide (Q\u2010NO) and 8\u2010hydroxyquinoline\u2010<jats:italic>N<\/jats:italic>\u2010oxide (HO\u2010Q\u2010NO) were used as model compounds on three different mass spectrometers (LCQ Deca, LCQ Advantage and TSQ Quantum). Irrespective of heated capillary or ion transfer tube length, <jats:italic>N<\/jats:italic>\u2010oxides from this class of compounds underwent predominantly deoxygenation decomposition under atmospheric pressure chemical ionization conditions and the abundance of the diagnostic [M + H \u2212 O]<jats:sup>+<\/jats:sup> ions increased with increasing vaporizer temperature. Furthermore, the results suggest that in API source decompostion methods described in this paper can be conducted using mass spectrometers with non\u2010heated capillary or ion transfer tube API interfaces. Because N\u2010oxides can undergo in\u2010source decomposition and interfere with quantitation experiments, particular attention should be paid when developing API based bioanalytical methods. Copyright \u00a9 2004 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/jms.623","type":"journal-article","created":{"date-parts":[[2004,6,30]],"date-time":"2004-06-30T03:00:51Z","timestamp":1088564451000},"page":"600-606","source":"Crossref","is-referenced-by-count":41,"title":["Distinguishing <i>N<\/i>\u2010oxide and hydroxyl compounds: impact of heated capillary\/heated ion transfer tube in inducing atmospheric pressure ionization source decompositions"],"prefix":"10.1002","volume":"39","author":[{"given":"Dilrukshi M.","family":"Peiris","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wing","family":"Lam","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Steven","family":"Michael","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ragu","family":"Ramanathan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,6,8]]},"reference":[{"key":"e_1_2_6_2_2","first-page":"275","article-title":"Improving drug R&D efficiency","author":"Siddiqui MA","year":"2001","journal-title":"Chem. 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